Plant Cold Acclimation

1. Front Matter

   Pages i-xii

   PDF

2. Introduction: Plant Cold Acclimation and Winter Survival

   • Dirk K. Hincha, Ellen Zuther

   Pages 1-7

3. Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays

   • Anja Thalhammer, Majken Pagter, Dirk K. Hincha, Ellen Zuther

   Pages 9-21

4. Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements

   • Andrey V. Malyshev, Ilka Beil, Juergen Kreyling

   Pages 23-31

5. Infrared Thermal Analysis of Plant Freezing Processes

   • Gilbert Neuner, Edith Lichtenberger

   Pages 33-41

6. Conducting Field Trials for Frost Tolerance Breeding in Cereals

   • Luigi Cattivelli, Cristina Crosatti

   Pages 43-52

7. A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes

   • Annick Bertrand, Annie Claessens, Josée Bourassa, Solen Rocher, Vern S. Baron

   Pages 53-60

8. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

   • Evelyne Téoulé, Carine Géry

   Pages 61-84

9. Identification of Arabidopsis Mutants with Altered Freezing Tolerance

   • Carlos Perea-Resa, Rafael Catalá, Julio Salinas

   Pages 85-97

10. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

    • Seizo Fujikawa, Keita Endoh

    Pages 99-117

11. Using Pixel-Based Microscope Images to Generate 3D Reconstructions of Frozen and Thawed Plant Tissue

    • David P. Livingston III, Tan D. Tuong

    Pages 119-139

12. Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography

    • Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, Karen K. Tanino

    Pages 141-159

13. Proteomic Approaches to Identify Proteins Responsive to Cold Stress

    • Anna M. Jozefowicz, Stefanie Döll, Hans-Peter Mock

    Pages 161-170

14. Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins

    • Md Mostafa Kamal, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, Matsuo Uemura

    Pages 171-186

15. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

    • Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura et al.

    Pages 187-202

16. Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

    • Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle et al.

    Pages 203-239

17. Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation

    • Andras Bittner, Thomas Griebel, Jörn van Buer, Ilona Juszczak-Debosz, Margarete Baier

    Pages 241-254

18. Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation

    • Daisuke Takahashi, Ellen Zuther, Dirk K. Hincha

    Pages 255-268

19. Subcellular Compartmentation of Metabolites Involved in Cold Acclimation

    • Imke I. Hoermiller, Thomas Nägele, Arnd G. Heyer

    Pages 269-275

20. Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime

    • Lisa Fürtauer, Thomas Nägele

    Pages 277-287

This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.

• Deacclimation
• Plant breeding
• Cold memory
• Protein analysis
• Gene expression
• Freezing tolerance

• Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

  Dirk K. Hincha, Ellen Zuther

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