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Plant Cold Acclimation

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Dirk K. Hincha

    1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Ellen Zuther

    1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2156)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Introduction: Plant Cold Acclimation and Winter Survival

    • Dirk K. Hincha, Ellen Zuther
    Pages 1-7

  3. Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays

    • Anja Thalhammer, Majken Pagter, Dirk K. Hincha, Ellen Zuther
    Pages 9-21

  4. Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements

    • Andrey V. Malyshev, Ilka Beil, Juergen Kreyling
    Pages 23-31

  5. Infrared Thermal Analysis of Plant Freezing Processes

    • Gilbert Neuner, Edith Lichtenberger
    Pages 33-41

  6. Conducting Field Trials for Frost Tolerance Breeding in Cereals

    • Luigi Cattivelli, Cristina Crosatti
    Pages 43-52

  7. A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes

    • Annick Bertrand, Annie Claessens, Josée Bourassa, Solen Rocher, Vern S. Baron
    Pages 53-60

  8. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

    • Evelyne Téoulé, Carine Géry
    Pages 61-84

  9. Identification of Arabidopsis Mutants with Altered Freezing Tolerance

    • Carlos Perea-Resa, Rafael Catalá, Julio Salinas
    Pages 85-97

  10. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

    • Seizo Fujikawa, Keita Endoh
    Pages 99-117

  11. Using Pixel-Based Microscope Images to Generate 3D Reconstructions of Frozen and Thawed Plant Tissue

    • David P. Livingston III, Tan D. Tuong
    Pages 119-139

  12. Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography

    • Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, Karen K. Tanino
    Pages 141-159

  13. Proteomic Approaches to Identify Proteins Responsive to Cold Stress

    • Anna M. Jozefowicz, Stefanie Döll, Hans-Peter Mock
    Pages 161-170

  14. Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins

    • Md Mostafa Kamal, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, Matsuo Uemura
    Pages 171-186

  15. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

    • Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura et al.
    Pages 187-202

  16. Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

    • Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle et al.
    Pages 203-239

  17. Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation

    • Andras Bittner, Thomas Griebel, Jörn van Buer, Ilona Juszczak-Debosz, Margarete Baier
    Pages 241-254

  18. Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation

    • Daisuke Takahashi, Ellen Zuther, Dirk K. Hincha
    Pages 255-268

  19. Subcellular Compartmentation of Metabolites Involved in Cold Acclimation

    • Imke I. Hoermiller, Thomas Nägele, Arnd G. Heyer
    Pages 269-275

  20. Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime

    • Lisa Fürtauer, Thomas Nägele
    Pages 277-287
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Keywords

About this book

This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.

Editors and Affiliations

  • Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha, Ellen Zuther

Bibliographic Information

  • Book Title: Plant Cold Acclimation

  • Book Subtitle: Methods and Protocols

  • Editors: Dirk K. Hincha, Ellen Zuther

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0660-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0659-9Published: 01 July 2020

  • Softcover ISBN: 978-1-0716-0662-9Published: 01 July 2021

  • eBook ISBN: 978-1-0716-0660-5Published: 30 June 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 336

  • Number of Illustrations: 28 b/w illustrations, 64 illustrations in colour

  • Topics: Plant Sciences, Plant Ecology, Plant Physiology

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