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Molecular Dermatology

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2154)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-x
  2. Isolation of Epidermal and Hair Follicle Melanocytes

    • Richard Baker, M. Julie Thornton
    Pages 23-32
  3. Isolation and Culture of Human Skin Mast Cells

    • Hanna Siiskonen, Jörg Scheffel
    Pages 33-43
  4. Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts

    • Isabelle Petit, Ayelet Levy, Daniel Aberdam
    Pages 63-72
  5. Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells

    • Ekaterina P. Kalabusheva, Ekaterina A. Vorotelyak
    Pages 153-163
  6. Identification of Long Noncoding RNA by In Situ Hybridization Approaches

    • Mara Mancini, Anna Maria Lena, Eleonora Candi
    Pages 175-185
  7. Detection of MicroRNAs by In Situ Hybridization in Skin

    • Maximilian E. Pickup, Mohammed I. Ahmed
    Pages 187-196
  8. Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells

    • Carmit Bar, V. Julian Valdes, Elena Ezhkova
    Pages 197-215
  9. 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells in Situ

    • Andrei N. Mardaryev, Michael Y. Fessing
    Pages 217-230

About this book

This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situin vitroex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.

Editors and Affiliations

  • Monasterium Laboratory Skin and Hair Research Solutions GmbH, Münster, Germany

    ​Natalia V. Botchkareva

  • Centre for Skin Sciences, University of Bradford, Bradford, UK

    Gillian E. Westgate

Bibliographic Information

  • Book Title: Molecular Dermatology

  • Book Subtitle: Methods and Protocols

  • Editors: ​Natalia V. Botchkareva, Gillian E. Westgate

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0648-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0647-6Published: 21 April 2020

  • Softcover ISBN: 978-1-0716-0650-6Published: 21 April 2021

  • eBook ISBN: 978-1-0716-0648-3Published: 20 April 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Number of Illustrations: 6 b/w illustrations, 69 illustrations in colour

  • Topics: Biochemistry, general, Dermatology, Cell Biology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access