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Intrinsically Disordered Proteins

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Birthe B. Kragelund

    1. Structural Biology and NMR Laboratory, Department of Biology, University of Copenhagen, København N, Denmark

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  2. Karen Skriver

    1. Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen, Denmark

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2141)

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Table of contents (47 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Sequence Properties

    1. Front Matter

      Pages 1-1
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    2. Disorder for Dummies: Functional Mutagenesis of Transient Helical Segments in Disordered Proteins

      • Gary W. Daughdrill
      Pages 3-20

    3. Computational Prediction of Intrinsic Disorder in Protein Sequences with the disCoP Meta-predictor

      • Christopher J. Oldfield, Xiao Fan, Chen Wang, A. Keith Dunker, Lukasz Kurgan
      Pages 21-35

    4. Computational Prediction of Disordered Protein Motifs Using SLiMSuite

      • Richard J. Edwards, Kirsti Paulsen, Carla M. Aguilar Gomez, Åsa Pérez-Bercoff
      Pages 37-72

    5. How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource

      • Marc Gouw, Jesús Alvarado-Valverde, Jelena Čalyševa, Francesca Diella, Manjeet Kumar, Sushama Michael et al.
      Pages 73-102

    6. Analyzing the Sequences of Intrinsically Disordered Regions with CIDER and localCIDER

      • Garrett M. Ginell, Alex S. Holehouse
      Pages 103-126

    7. Exploring Protein Intrinsic Disorder with MobiDB

      • Alexander Miguel Monzon, András Hatos, Marco Necci, Damiano Piovesan, Silvio C. E. Tosatto
      Pages 127-143

  3. Evolution

    1. Front Matter

      Pages 145-145
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    2. An Easy Protocol for Evolutionary Analysis of Intrinsically Disordered Proteins

      • Janelle Nunez-Castilla, Jessica Siltberg-Liberles
      Pages 147-177

  4. Production

    1. Front Matter

      Pages 179-179
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    2. Expression and Purification of an Intrinsically Disordered Protein

      • Karamjeet K. Singh, Steffen P. Graether
      Pages 181-194

    3. Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricks

      • Christian Parsbæk Pedersen, Pernille Seiffert, Inna Brakti, Katrine Bugge
      Pages 195-209

    4. Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond

      • Albert Escobedo, Giulio Chiesa, Xavier Salvatella
      Pages 211-231

    5. Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy

      • Linnéa Isaksson, Anders Pedersen
      Pages 233-245

  5. Dynamics, Ensembles, and Structures

    1. Front Matter

      Pages 247-247
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    2. Structural Analyses of Intrinsically Disordered Proteins by Small-Angle X-Ray Scattering

      • Amin Sagar, Dmitri Svergun, Pau Bernadó
      Pages 249-269

    3. Determining Rg of IDPs from SAXS Data

      • Ellen Rieloff, Marie Skepö
      Pages 271-283

    4. Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements

      • Sarah Leeb, Jens Danielsson
      Pages 285-302

    5. Quantitative Protein Disorder Assessment Using NMR Chemical Shifts

      • Jakob T. Nielsen, Frans A. A. Mulder
      Pages 303-317
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Keywords

About this book

The edition details methods to study intrinsically disordered proteins (IDPs) including recent topics such as extremely high-affinity disordered complexes, kinetics that evade established concepts, liquid-liquid phase separation, and novel disorder-driven allosteric mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Intrinsically Disordered Proteins: Methods and Protocols aims to help scientists with different backgrounds to further their investigations into these fascinating and dynamic molecules.

Chapter 24 is available open access under a CC BY 4.0 license via link.springer.com.

Chapters “40 and 42 ” are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Editors and Affiliations

  • Structural Biology and NMR Laboratory, Department of Biology, University of Copenhagen, København N, Denmark

    Birthe B. Kragelund

  • Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen, Denmark

    Karen Skriver

Bibliographic Information

  • Book Title: Intrinsically Disordered Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Birthe B. Kragelund, Karen Skriver

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0524-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0523-3Published: 22 July 2020

  • Softcover ISBN: 978-1-0716-0526-4Published: 23 July 2021

  • eBook ISBN: 978-1-0716-0524-0Published: 21 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 951

  • Number of Illustrations: 39 b/w illustrations, 192 illustrations in colour

  • Topics: Protein Science

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