Skip to main content
SpringerLink
Log in

Lectin Purification and Analysis

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Jun Hirabayashi

    1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2132)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 239.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (60 protocols)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Structural Database for Lectins and the UniLectin Web Platform

    • François Bonnardel, Serge Perez, Frédérique Lisacek, Anne Imberty
    Pages 1-14

  3. Purification of Sugar-Binding Peptides from L-Type Lectins

    • Kazuo Yamamoto
    Pages 15-20

  4. Recombinant Expression and Purification of Animal Intracellular L-Type Lectins

    • Tadashi Satoh, Koichi Kato
    Pages 21-28

  5. Frontal Affinity Chromatography: A Highly Suitable Retardation Phenomenon-Based Research Tool for Analyzing Weak Interactions Between Biomolecules

    • Kenichi Kasai
    Pages 29-37

  6. Metazoan Soluble β-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of Their Carbohydrate-Binding Specificity, and Probing Their Ligands

    • Guillaume St-Pierre, Ann Rancourt, Sachiko Sato
    Pages 39-54

  7. Expression, S-Nitrosylation, and Measurement of S-Nitrosylation Ratio of Recombinant Galectin-2

    • Mayumi Tamura, Yoichiro Arata
    Pages 55-63

  8. Expression and Purification of Full-Length and Domain-Fragment Recombinant Pentraxin 3 (PTX3) Proteins from Mammalian and Bacterial Cells

    • Kenji Daigo, Takao Hamakubo
    Pages 65-74

  9. Identification of Siglec Cis-Ligands by Proximity Labeling

    • Amin Alborzian Deh Sheikh, Chizuru Akatsu, Takeshi Tsubata
    Pages 75-83

  10. Preparation of Recombinant Siglecs and Identification of Their Ligands

    • Lan-Yi Chang, Penk Yeir Low, Deepa Sridharan, Kaia Gerlovin, Takashi Angata
    Pages 85-98

  11. Purification, Quantification, and Functional Analysis of Collectins

    • Katsuki Ohtani, Nobutaka Wakamiya
    Pages 99-109

  12. Selectin-Binding Assay by Flow Cytometry

    • Keiichiro Sakuma, Reiji Kannagi
    Pages 111-118

  13. Direct Binding Analysis Between C-Type Lectins and Glycans Using Immunoglobulin Receptor Fusion Proteins

    • Miyuki Watanabe, Zakaria Omahdi, Sho Yamasaki
    Pages 119-128

  14. Expression and Characterization of Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization: HYBID, Alias KIAA1199 and CEMIP

    • Susana de Vega, Hiroyuki Yoshida, Yasunori Okada
    Pages 129-138

  15. Paracoccin: Purification and Validation of Its Lectin and Enzymatic Properties

    • Nayla de Souza Pitangui, Fabrício Freitas Fernandes, Relber Aguiar Gonçales, Maria Cristina Roque-Barreira
    Pages 139-149

  16. In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing α-Mannosidase-Like Proteins

    • Nobuko Hosokawa
    Pages 151-158

  17. Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria echinata

    • Tomomitsu Hatakeyama
    Pages 159-164

  18. Simple and Rapid Detection of Glycoforms by “Lectin Inhibition” Assay

    • Kyoka Hoshi, Yasuhiro Hashimoto, Hiromi Ito
    Pages 165-171

  19. Glycoform-Specific Visualization in Immunohistochemistry by “Lectin Inhibition”

    • Hiromi Ito, Kyoka Hoshi, Yasuhiro Hashimoto, Takashi Honda
    Pages 173-181

  20. Botulinum Hemagglutinin: Critical Protein for Adhesion and Absorption of Neurotoxin Complex in Host Intestine

    • Sho Amatsu, Yukako Fujinaga
    Pages 183-190
Back to top

Keywords

About this book

This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.



Editors and Affiliations

  • Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

Bibliographic Information

  • Book Title: Lectin Purification and Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Jun Hirabayashi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0430-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0429-8Published: 18 April 2020

  • Softcover ISBN: 978-1-0716-0432-8Published: 18 April 2021

  • eBook ISBN: 978-1-0716-0430-4Published: 18 April 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 699

  • Number of Illustrations: 82 b/w illustrations, 110 illustrations in colour

  • Topics: Protein Science, Plant Sciences

Publish with us

Policies and ethics

Back to top

Search

Navigation