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  • © 2020

Neurohistology and Imaging Techniques

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 153)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiv
  2. Neurohistology with a Touch of History: Technology-Driven Research

    • Floris G. Wouterlood, Thomas P. Langer
    Pages 1-48
  3. Fixation Protocols for Neurohistology: Neurons to Genes

    • Elliott J. Mufson, Sylvia E. Perez, Christy M. Kelley, Melissa J. Alldred, Stephen D. Ginsberg
    Pages 49-71
  4. Three-Dimensional Atlases of Insect Brains

    • Basil el Jundi, Stanley Heinze
    Pages 73-124
  5. Optical Imaging Probes for Amyloid Diseases in Brain

    • Pratyush Kumar Mishra, Myeong-Gyun Kang, Hyun-Woo Rhee
    Pages 157-182
  6. Chemical Clearing of GFP-Expressing Neural Tissues

    • Klaus Becker, Saiedeh Saghafi, Christian Hahn, Nina Jährling, Hans-Ulrich Dodt
    Pages 183-199
  7. Stereomicroscopy in Neuroanatomy

    • Erin E. Wilson, William Chambers, Radek Pelc, Paul Nothnagle, Michael W. Davidson
    Pages 245-274
  8. Conventional, Apodized, and Relief Phase-Contrast Microscopy

    • Radek Pelc, Zdeněk Hostounský, Tatsuro Otaki, Kaoru Katoh
    Pages 275-323
  9. Ultramicroscopy of Nerve Fibers and Neurons: Fine-Tuning the Light Sheets

    • Saiedeh Saghafi, Klaus Becker, Nina Jährling, Christian Hahn, Hans-Ulrich Dodt
    Pages 325-339
  10. Imaging and Electrophysiology of Individual Neurites Functionally Isolated in Microchannels

    • Heinz D. Wanzenboeck, Petra Scholze, Johann K. Mika
    Pages 341-377
  11. Digital Micrographs in Pathology

    • Roger S. Riley, Jorge Almenara, Christine E. Fuller
    Pages 403-458
  12. Correction to: Neurohistology and Imaging Techniques

    • Radek Pelc, Wolfgang Walz, J. Ronald Doucette
    Pages C1-C1
  13. Back Matter

    Pages 459-472

About this book

This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-­expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.                        

Editors and Affiliations

  • Czech Academy of Sciences and Charles University, Prague, Czech Republic

    Radek Pelc

  • Department of Psychiatry, University of Saskatchewan, Saskatoon, Canada

    Wolfgang Walz

  • Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Canada

    J. Ronald Doucette

Bibliographic Information

  • Book Title: Neurohistology and Imaging Techniques

  • Editors: Radek Pelc, Wolfgang Walz, J. Ronald Doucette

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-0428-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2020

  • Hardcover ISBN: 978-1-0716-0426-7Published: 20 November 2020

  • eBook ISBN: 978-1-0716-0428-1Published: 29 July 2020

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 472

  • Number of Illustrations: 54 b/w illustrations, 105 illustrations in colour

  • Topics: Neurosciences

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access