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Immunity in Insects

  • Book
  • © 2020

Overview

Editors:
  1. Federica Sandrelli

    1. Department of Biology, University of Padova, Padova, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Gianluca Tettamanti

    1. Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  2. Searching for Genes and Proteins Involved in the Immune Response

    1. Front Matter

      Pages 1-1
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    2. Characterization of Insect Immune Systems from Genomic Data

      • Robert M. Waterhouse, Brian P. Lazzaro, Timothy B. Sackton
      Pages 3-34

    3. Analyzing Immunity in Non-model Insects Using De Novo Transcriptomics

      • Shulin He, Paul R. Johnston, Dino P. McMahon
      Pages 35-51

    4. Preparation of Insect Protein Samples for Label-Free Proteomic Quantification by LC-Mass Spectrometry

      • Alexandro Rodríguez-Rojas, Jens Rolff
      Pages 53-61

  3. Cellular Response

    1. Front Matter

      Pages 63-63
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    2. Quantification of Blood Cells in Drosophila melanogaster and Other Insects

      • Sean Corcoran, Katja Brückner
      Pages 65-77

    3. Methods to Quantify In Vivo Phagocytic Uptake and Opsonization of Live or Killed Microbes in Drosophila melanogaster

      • Samuel Liégeois, Wenhui Wang, Dominique Ferrandon
      Pages 79-95

    4. Analysis of Cellular Immune Responses in Lepidopteran Larvae

      • Andrea Becchimanzi, Ilaria Di Lelio, Francesco Pennacchio, Silvia Caccia
      Pages 97-111

  4. Humoral Response: Melanization, Lysozyme, and Antimicrobial Peptides

    1. Front Matter

      Pages 113-113
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    2. Detection of Enzyme Distribution, Expression, Activation, and Activity of Insect Prophenoloxidase

      • Kai Wu, Bing Yang, Jing Wang, Yufa Luo, Yuyang Ni, Wuren Huang
      Pages 115-126

    3. Basic Methods to Evaluate Humoral Immunity Processes in Lepidoptera Larvae

      • Maristella Mastore, Maurizio Francesco Brivio
      Pages 127-144

  5. Humoral Response: Antimicrobial Peptides as Alternatives to Conventional Antibiotics

    1. Front Matter

      Pages 145-145
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    2. Methods for the In Vitro Examination of the Antibacterial and Cytotoxic Activities of Antimicrobial Peptides

      • Bruno Casciaro, Floriana Cappiello, Maria Rosa Loffredo, Maria Luisa Mangoni
      Pages 147-162

    3. Characterization of Antimicrobial Peptide–Membrane Interaction Using All-Atom Molecular Dynamic Simulation

      • Shruti Mukherjee, Rajiv K. Kar, Anirban Bhunia
      Pages 163-176

  6. Insect Immunity and Environmental Interactions

    1. Front Matter

      Pages 177-177
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    2. Manipulating the Mosquito Microbiota to Study Its Function

      • Ottavia Romoli, Mathilde Gendrin
      Pages 179-189

    3. Discovery and Analysis of RNA Viruses in Insects

      • Lumi Viljakainen, Jaana Jurvansuu
      Pages 191-200

  7. Infection Models

    1. Front Matter

      Pages 201-201
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    2. A Workflow for Infection of Drosophila with Entomopathogenic Nematodes to Monitor Immune Gene Transcriptional Activity

      • Christa Heryanto, Eric Kenney, Ioannis Eleftherianos
      Pages 203-216
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Keywords

About this book

This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Immunity in Insects aims to ensure successful resultsin the further study of this vital field.

Reviews

“With its broad and lucid treatment of insect immunity, this volume paves the way for new recruits and encourages current researchers to expand and refine their approaches.” (James B. Nardi, The Quarterly Review of Biology, Vol. 95 (4), December, 2020)

Editors and Affiliations

  • Department of Biology, University of Padova, Padova, Italy

    Federica Sandrelli

  • Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy

    Gianluca Tettamanti

Bibliographic Information

  • Book Title: Immunity in Insects

  • Editors: Federica Sandrelli, Gianluca Tettamanti

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-0259-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0258-4Published: 28 January 2020

  • Softcover ISBN: 978-1-0716-0261-4Published: 28 January 2021

  • eBook ISBN: 978-1-0716-0259-1Published: 27 January 2020

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XI, 246

  • Number of Illustrations: 7 b/w illustrations, 43 illustrations in colour

  • Topics: Immunology, Entomology

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