Overview
- Editors:
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Ralf Pörtner
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Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, Germany
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (22 protocols)
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Cell Line and Media Development
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- Johannes Möller, Katrin Korte, Ralf Pörtner, An-Ping Zeng, Uwe Jandt
Pages 3-16
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- Oliver Wehmeier, Alexander Loa
Pages 17-25
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- Patrick Mayrhofer, Renate Kunert
Pages 27-39
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Techniques for Process Development
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- Vincent Wiegmann, Cristina Bernal Martinez, Frank Baganz
Pages 69-81
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- Tibor Anderlei, Michael V. Keebler, Jordi Joan CairĂł, MartĂ Lecina
Pages 83-103
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- Tim BĂĽrgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl et al.
Pages 105-123
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- Moritz Wolf, Massimo Morbidelli
Pages 125-140
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- Alexander Nikolay, Thomas Bissinger, Gwendal Gränicher, Yixiao Wu, Yvonne Genzel, Udo Reichl
Pages 141-168
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- Cedric Schirmer, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl, Dieter Eibl
Pages 169-186
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Model-Based Techniques for Process Development
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Front Matter
Pages 187-187
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- Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, Christoph Herwig
Pages 189-211
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- Sahar Deppe, Björn Frahm, Volker C. Hass, Tanja Hernández RodrĂguez, Kim B. KuchemĂĽller, Johannes Möller et al.
Pages 213-234
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- Kim B. Kuchemüller, Ralf Pörtner, Johannes Möller
Pages 235-249
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- Tanja Hernández RodrĂguez, Björn Frahm
Pages 251-267
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Process Analysis
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Front Matter
Pages 269-269
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About this book
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Editors and Affiliations
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Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, Germany
Ralf Pörtner