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Animal Cell Biotechnology

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Ralf Pörtner

    1. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2095)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cell Line and Media Development

    1. Front Matter

      Pages 1-1
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    2. Near-Physiological Cell Cycle Synchronization with Countercurrent Centrifugal Elutriation

      • Johannes Möller, Katrin Korte, Ralf Pörtner, An-Ping Zeng, Uwe Jandt
      Pages 3-16

    3. Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays

      • Oliver Wehmeier, Alexander Loa
      Pages 17-25

    4. Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment

      • Patrick Mayrhofer, Renate Kunert
      Pages 27-39

  3. Techniques for Process Development

    1. Front Matter

      Pages 41-41
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    2. ambr® 15 Microbioreactors for CHO Cell Cultivation

      • Steve R. C. Warr
      Pages 43-67

    3. Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications

      • Vincent Wiegmann, Cristina Bernal Martinez, Frank Baganz
      Pages 69-81

    4. HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR)

      • Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, Martí Lecina
      Pages 83-103

    5. Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode

      • Tim Bürgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl et al.
      Pages 105-123

    6. Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors

      • Moritz Wolf, Massimo Morbidelli
      Pages 125-140

    7. Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production

      • Alexander Nikolay, Thomas Bissinger, Gwendal Gränicher, Yixiao Wu, Yvonne Genzel, Udo Reichl
      Pages 141-168

    8. How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale

      • Cedric Schirmer, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl, Dieter Eibl
      Pages 169-186

  4. Model-Based Techniques for Process Development

    1. Front Matter

      Pages 187-187
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    2. Generic Workflow for the Setup of Mechanistic Process Models

      • Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, Christoph Herwig
      Pages 189-211

    3. Estimation of Process Model Parameters

      • Sahar Deppe, Björn Frahm, Volker C. Hass, Tanja Hernández Rodríguez, Kim B. Kuchemüller, Johannes Möller et al.
      Pages 213-234

    4. Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments

      • Kim B. Kuchemüller, Ralf Pörtner, Johannes Möller
      Pages 235-249

    5. Design, Optimization, and Adaptive Control of Cell Culture Seed Trains

      • Tanja Hernández Rodríguez, Björn Frahm
      Pages 251-267

  5. Process Analysis

    1. Front Matter

      Pages 269-269
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    2. High-Throughput Quantification and Glycosylation Analysis of Antibodies Using Bead-Based Assays

      • Sebastian Giehring
      Pages 271-284
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Keywords

About this book

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.




Editors and Affiliations

  • Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, Germany

    Ralf Pörtner

Bibliographic Information

  • Book Title: Animal Cell Biotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Ralf Pörtner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0191-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0190-7Published: 20 December 2019

  • Softcover ISBN: 978-1-0716-0193-8Published: 17 January 2021

  • eBook ISBN: 978-1-0716-0191-4Published: 19 December 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XIV, 399

  • Number of Illustrations: 47 b/w illustrations, 80 illustrations in colour

  • Topics: Biotechnology, Pharmaceutical Sciences/Technology

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