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Inositol Phosphates

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Gregory J. Miller

    1. Department of Chemistry, The Catholic University of America, Washington, DC, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2091)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. A Short Historical Perspective of Methods in Inositol Phosphate Research

    • Stephen B. Shears
    Pages 1-28

  3. Isolation and Measurement

    1. Front Matter

      Pages 29-29
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    2. Quantitation of Inositol Phosphates by HPLC-ESI-MS

      • Quynh H. Duong, Ronald B. Pegg
      Pages 31-37

    3. Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation

      • Benjamin L. Turner
      Pages 39-46

    4. Detection of Inositol Phosphates by Split PH Domains

      • Reiko Sakaguchi, Shunsuke Tajima, Yasuo Mori, Takashi Morii
      Pages 47-57

    5. Dictyostelium discoideum as a Model to Study Inositol Polyphosphates and Inorganic Polyphosphate

      • Yann Desfougères, Adolfo Saiardi
      Pages 59-71

  4. Assays for Function

    1. Front Matter

      Pages 73-73
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    2. Characterizing Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family

      • Lucinda Winward, Rajagopal Sharada Kilari, Stephen T. Safrany
      Pages 75-82

    3. Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells

      • Andrew T. Hale, Bradley P. Clarke, John D. York
      Pages 83-92

    4. Back-Pyrophosphorylation Assay to Detect In Vivo InsP7-Dependent Protein Pyrophosphorylation in Mammalian Cells

      • Manasa Chanduri, Rashna Bhandari
      Pages 93-105

    5. Analyses of Ligand Binding to IP3 Receptors Using Fluorescence Polarization

      • Ana M. Rossi, Colin W. Taylor
      Pages 107-120

    6. Investigating the InsP3 Receptor in Living Cells by Caged InsP3

      • Xin Hui, Peter Lipp
      Pages 121-129

    7. Optical Measurement of Synaptic Vesicle Fusion and Its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons

      • Sung Hyun Kim
      Pages 131-135

    8. Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate

      • Akihiko Tanimura, Satoshi Shuto
      Pages 137-144

    9. Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle–Vesicle Fusion Assay

      • Mi Sook Sung, Tae-Young Yoon
      Pages 145-152

  5. Chemical Tools

    1. Front Matter

      Pages 153-153
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    2. Engineering Protease-Resistant and Highly Active Phytases

      • Canfang Niu, Peilong Yang, Bin Yao
      Pages 155-162

    3. Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-Cleaving Tag

      • Keehwan Kwon, Marco Biancucci, Patrick J. Woida, Karla J. F. Satchell
      Pages 163-179

  6. Back Matter

    Pages 181-185
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Keywords

About this book

This volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Chemistry, The Catholic University of America, Washington, DC, USA

    Gregory J. Miller

Bibliographic Information

  • Book Title: Inositol Phosphates

  • Book Subtitle: Methods and Protocols

  • Editors: Gregory J. Miller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0167-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0166-2Published: 27 November 2019

  • Softcover ISBN: 978-1-0716-0169-3Published: 27 November 2020

  • eBook ISBN: 978-1-0716-0167-9Published: 26 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 185

  • Number of Illustrations: 20 b/w illustrations, 15 illustrations in colour

  • Topics: Biochemistry, general, Cell Biology

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