Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2087)
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Table of contents (26 protocols)
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Front Matter
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Neutrophils and Neutrophil Disorders: Overviews
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Front Matter
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Neutrophil Chemotaxis, Phagocytosis, and Bactericidal Activity
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Front Matter
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Biochemistry, Biology, and Signal Transduction of Neutrophils
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Front Matter
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About this book
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
Features
• Provides neutrophil protocols that are broadly applicable to leukocyte biology
• Includes protocols for isolation of neutrophils from humans and animal species
• Presents an interesting and informative overview of the field of neutrophil biology
• Facilitates a better understanding of the role of neutrophils in host defense
Readership
Molecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists
Editors and Affiliations
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Department of Microbiology and Immunology, Montana State University, Bozeman, USA
Mark T. Quinn
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Laboratory of Bacteriology, Rocky Mountain Laboratories Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, USA
Frank R. DeLeo
Bibliographic Information
Book Title: Neutrophil
Book Subtitle: Methods and Protocols
Editors: Mark T. Quinn, Frank R. DeLeo
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0154-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0153-2Published: 14 November 2019
Softcover ISBN: 978-1-0716-0156-3Published: 14 November 2020
eBook ISBN: 978-1-0716-0154-9Published: 14 November 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XVI, 470
Number of Illustrations: 37 b/w illustrations, 49 illustrations in colour
Topics: Immunology, Cell Biology