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Chimeric Antigen Receptor T Cells

Development and Production

  • Book
  • © 2020

Overview

Editors:
  1. Kamilla Swiech

    1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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  2. Kelen Cristina Ribeiro Malmegrim

    1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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  3. Virgínia Picanço-Castro

    1. Center for Cell-Based Therapy CTC Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2086)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. CAR-T Cells for Cancer Treatment: Current Design and Next Frontiers

    • Virgínia Picanço-Castro, Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Dimas Tadeu Covas
    Pages 1-10

  3. Design of Chimeric Antigen Receptors and Vector Production

    1. Front Matter

      Pages 11-11
      Download chapter PDF

    2. Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries

      • Nestor F. Leyton-Castro, Marcelo M. Brigido, Andrea Q. Maranhão
      Pages 13-26

    3. Phase I/II Manufacture of Lentiviral Vectors Under GMP in an Academic Setting

      • Anindya Dasgupta, Stuart Tinch, Kathleen Szczur, Rebecca Ernst, Nathaniel Shryock, Courtney Kaylor et al.
      Pages 27-60

    4. Production of Lentivirus for the Establishment of CAR-T Cells

      • Marlous G. Lana, Bryan E. Strauss
      Pages 61-67

    5. Optimized Production of Lentiviral Vectors for CAR-T Cell

      • Pablo Diego Moço, Mário Soares de Abreu Neto, Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro
      Pages 69-76

    6. Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells

      • Aline Do Minh, Michelle Yen Tran, Amine A. Kamen
      Pages 77-83

  4. CAR-T Cells Generation and Manufacturing

    1. Front Matter

      Pages 85-85
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    2. In Vitro-Transcribed (IVT)-mRNA CAR Therapy Development

      • Androulla N. Miliotou, Lefkothea C. Papadopoulou
      Pages 87-117

    3. Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors

      • Feiyan Mo, Maksim Mamonkin
      Pages 119-130

    4. Generation of CAR+ T Lymphocytes Using the Sleeping Beauty Transposon System

      • Leonardo Chicaybam, Luiza Abdo, Martín H. Bonamino
      Pages 131-137

    5. Platforms for Clinical-Grade CAR-T Cell Expansion

      • Amanda Mizukami, Kamilla Swiech
      Pages 139-150

    6. CAR-T Cell Expansion in a Xuri Cell Expansion System W25

      • Trevor A. Smith
      Pages 151-163

    7. Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform

      • Josh Ludwig, Mark Hirschel
      Pages 165-177

  5. CAR-T Cells Characterization and Quality Control

    1. Front Matter

      Pages 179-179
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    2. Replication-Competent Lentivirus Analysis of Vector-Transduced T Cell Products Used in Cancer Immunotherapy Clinical Trials

      • Kenneth Cornetta, Sue Koop, Emily Nance, Kimberley House, Lisa Duffy
      Pages 181-194

    3. Immunophenotypic Analysis of CAR-T Cells

      • Júlia Teixeira Cottas de Azevedo, Amanda Mizukami, Pablo Diego Moço, Kelen Cristina Ribeiro Malmegrim
      Pages 195-201

    4. Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies

      • Robert D. Schwab, Darel Martínez Bedoya, Tiffany R. King, Bruce L. Levine, Avery D. Posey Jr.
      Pages 203-211

    5. Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays

      • Renata Nacasaki Silvestre, Pablo Diego Moço, Virgínia Picanço-Castro
      Pages 213-222
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Keywords

About this book

This volume provides comprehensive methods from expert scientists working in the  Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.

Editors and Affiliations

  • School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim

  • Center for Cell-Based Therapy CTC Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Virgínia Picanço-Castro

Bibliographic Information

  • Book Title: Chimeric Antigen Receptor T Cells

  • Book Subtitle: Development and Production

  • Editors: Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Virgínia Picanço-Castro

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0146-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0145-7Published: 10 November 2019

  • Softcover ISBN: 978-1-0716-0148-8Published: 10 November 2020

  • eBook ISBN: 978-1-0716-0146-4Published: 09 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 274

  • Number of Illustrations: 4 b/w illustrations, 25 illustrations in colour

  • Topics: Cancer Research, Cell Biology, Immunology, Pharmacology/Toxicology

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