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Bacterial Artificial Chromosomes

Volume 1: Library Construction, Physical Mapping, and Sequencing

  • Book
  • © 2004

Overview

Editors:
  1. Shaying Zhao

    1. The Institute for Genomic Research, Rockville

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Marvin Stodolsky

    1. Office of Biological and Environmental Research, US Department of Energy, Germantown

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 255)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xx
    Download chapter PDF

  2. BAC Library Construction

    • Kazutoyo Osoegawa, Pieter J. de Jong
    Pages 1-46

  3. Construction of Small Genome BAC Library for Functional and Genomic Applications

    • David Šmajs, Steven J. Norris, George M. Weinstock
    Pages 47-56

  4. Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging

    • Sangita Pal, Solida Mak, George M. Weinstock
    Pages 57-68

  5. Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions

    • Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, Vladimir Larionov
    Pages 69-89

  6. Purification of BAC DNA

    • Tim S. Poulsen
    Pages 91-100

  7. Hybridization-Based Selection of BAC Clones

    • Chang-Su Lim, Ung-Jin Kim
    Pages 101-111

  8. Applications of Interspersed Repeat Sequence Polymerase Chain Reaction

    • Heike Zimdahl, Claudia Gösele, Thomas Kreitler, Margit Knoblauch
    Pages 113-129

  9. BAC Mapping Using Fluorescence In Situ Hybridization

    • Xiao-Ning Chen, Julie R. Korenberg
    Pages 131-141

  10. High-Throughput BAC Fingerprinting

    • Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, Marco Marra
    Pages 143-156

  11. BAC End Sequencing

    • Tim S. Poulsen, Hans E. Johnsen
    Pages 157-161

  12. Radiation Hybrid Mapping With BAC Ends

    • Michael Olivier, Shannon Brady, David R. Cox
    Pages 163-169

  13. Shotgun Library Construction for DNA Sequencing

    • Bruce A. Roe
    Pages 171-187

  14. Rolling Circle Amplification for Sequencing Templates

    • Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina et al.
    Pages 189-196

  15. Transposon-Mediated Sequencing

    • Rachel Reeg, Anup Madan
    Pages 197-210

  16. Pyrosequencing

    • Elahe Elahi, Mostafa Ronaghi
    Pages 211-219

  17. Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions

    • Andrei Malykh, Nikolai Polushin, Alexei Slesarev, Sergei Kozyavkin
    Pages 221-229

  18. Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions

    • Pradeep K. Chatterjee
    Pages 231-241

  19. Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers

    • Pradeep K. Chatterjee, Joseph C. Baker Jr.
    Pages 243-254

  20. BAC Finishing Strategies

    • Christine Bird, Darren Grafham
    Pages 255-277
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Keywords

About this book

For both volumes:

Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Editors and Affiliations

  • The Institute for Genomic Research, Rockville

    Shaying Zhao

  • Office of Biological and Environmental Research, US Department of Energy, Germantown

    Marvin Stodolsky

Bibliographic Information

  • Book Title: Bacterial Artificial Chromosomes

  • Book Subtitle: Volume 1: Library Construction, Physical Mapping, and Sequencing

  • Editors: Shaying Zhao, Marvin Stodolsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597521

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-0-89603-988-9Published: 04 March 2004

  • Softcover ISBN: 978-1-61737-288-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-752-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 360

  • Number of Illustrations: 71 b/w illustrations

  • Topics: Bacteriology, Microbiology, Biochemistry, general

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