The continued successes of large- and small-scale genome sequencing projects are increasing the number of genomic targets available for drug d- covery at an exponential rate. In addition, a better understanding of molecular mechanisms—such as apoptosis, signal transduction, telomere control of ch- mosomes, cytoskeletal development, modulation of stress-related proteins, and cell surface display of antigens by the major histocompatibility complex m- ecules—has improved the probability of identifying the most promising genomic targets to counteract disease. As a result, developing and optimizing lead candidates for these targets and rapidly moving them into clinical trials is now a critical juncture in pharmaceutical research. Recent advances in com- natorial library synthesis, purification, and analysis techniques are not only increasing the numbers of compounds that can be tested against each specific genomic target, but are also speeding and improving the overall processes of lead discovery and optimization. There are two main approaches to combinatorial library production: p- allel chemical synthesis and split-and-mix chemical synthesis. These approaches can utilize solid- or solution-based synthetic methods, alone or in combination, although the majority of combinatorial library synthesis is still done on solid support. In a parallel synthesis, all the products are assembled separately in their own reaction vessels or microtiter plates. The array of rows and columns enables researchers to organize the building blocks to be c- bined, and provides an easy way to identify compounds in a particular well.

Reviews

"...an excellent work that covers many important aspects, gives easy access to literature for further study, and contains much practical advice. As it describes many different tools and techniques it is especially suitable for beginners in combinatorial chemistry, and deserves to be added to the list of well presented works in this field." - Angewandte Chemie

"The book provides a wealth of excellent reference material and it is a worthwhile addition to the library of most medicinal chemists." -Journal of Medicinal Chemistry

Editors and Affiliations

BD Biosciences, San Jose

Lisa Bellavance English

Bibliographic Information

Book Title: Combinatorial Library
Book Subtitle: Methods and Protocols
Editors: Lisa Bellavance English
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1592592856
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2002
Hardcover ISBN: 978-0-89603-980-3Published: 22 August 2002
Softcover ISBN: 978-1-4899-3899-2Published: 14 September 2013
eBook ISBN: 978-1-59259-285-2Published: 04 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 383
Topics: Biochemistry, general

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Table of contents (21 protocols)

Front Matter

Library Synthesis and Quality Control

Front Matter

Library Purification and Screening

Front Matter

Computational Library Design

Front Matter

About this book

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Editors and Affiliations

BD Biosciences, San Jose

Bibliographic Information

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