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Mitochondrial DNA

Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. William C. Copeland

    1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health

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Part of the book series: Methods in Molecular Biology (MIMB, volume 197)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Methods for the Analysis of MTDNA

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Animal Models for Mitochondrial Disease

      • Douglas C. Wallace
      Pages 3-54

    3. Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases

      • Eric A. Schon, Ali Naini, Sara Shanske
      Pages 55-74

    4. Screening for Aging-Dependent Point Mutations in mtDNA

      • Yuichi Michikawa, Giuseppe Attardi
      Pages 75-92

    5. Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis

      • Weiming Zheng, Luisa A. Marcelino, William G. Thilly
      Pages 93-106

    6. Identification of mtDNA Mutations in Human Cancer

      • Shuji Nomoto, Montserrant Sanchez-Cespedes, David Sidransky
      Pages 107-118

    7. In Situ PCR of the Common Human mtDNA Deletion

      • Steven J. Zullo
      Pages 119-128

    8. Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCyclerâ„¢

      • Alice Wong, Gino Cortopassi
      Pages 129-138

    9. Yeast Nuclear Genes for mtDNA Maintenance

      • Françoise Foury
      Pages 139-149

    10. Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay

      • Micheline K. Strand, William C. Copeland
      Pages 151-157

    11. Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR

      • Janine H. Santos, Bhaskar S. Mandavilli, Bennett Van Houten
      Pages 159-176

    12. Migration of mtDNA into the Nucleus

      • Mary K. Thorsness, Karen H. White, Peter E. Thorsness
      Pages 177-186

    13. 2D Gel Electrophoresis of mtDNA

      • Heather E. Lorimer
      Pages 187-196

  3. Methods for the Analysis of proteins involved in MTDNA Transactions

    1. Front Matter

      Pages 197-197
      Download chapter PDF

    2. Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions

      • Daniel F. Bogenhagen
      Pages 199-210

    3. Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography

      • Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, Dale W. Mosbaugh
      Pages 211-225

    4. Characterization of Specialized mtDNA Glycosylases

      • Simon G. Nyaga, Vilhelm A. Bohr
      Pages 227-244

    5. Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit

      • Matthew J. Longley, William C. Copeland
      Pages 245-257

    6. Assay of mtDNA Polymerase γ from Human Tissues

      • Robert K. Naviaux
      Pages 259-271
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About this book

Mutations within mitochondrial DNA (mtDNA) and the nuclear genes involved in the maintenance of mitochondrial DNA have been linked to a wide range of human diseases, including several of the most common diseases of aging. In Mitochondrial DNA: Methods and Protocols internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Each readily reproducible protocol includes step-by-step instructions, tips on avoiding pitfalls and extending the method to other situation, and introductory material explaining the theory behind the process.
Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing the role mtDNA plays in the aging process, apoptosis, and possibly some cancers, and for investigating the cause of mitochondrial dysfunction and disease.

Reviews

"Accordingly, the book is timely and of significant use to investigators working in this area. The book is divided into two major sections -- the first deals with analysis of the DNA and the second with analysis of related proteins. A valuable introductory chapter discusses available animal models for mitochondrial disease and the book concludes with a discussion of knockout mice. Given the general interest in mitochondrial mutations as they relate to a variety of human diseases, this contribution is timely and of value. The protocols are sufficiently detailed to allow adaptation into any working laboratory."-Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health

    William C. Copeland

Bibliographic Information

  • Book Title: Mitochondrial DNA

  • Book Subtitle: Methods and Protocols

  • Editors: William C. Copeland

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592848

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2002

  • eBook ISBN: 978-1-59259-284-5Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 414

  • Number of Illustrations: 120 b/w illustrations, 8 illustrations in colour

  • Topics: Cell Biology

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