Skip to main content
SpringerLink
Log in
Book cover

The Protein Protocols Handbook

  • Book
  • © 2002

Overview

Editors:
  1. John M. Walker

    1. University of Hertfordshire, Hatfield, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Protocols Handbooks (SPH)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (164 protocols)

  1. Front Matter

    Pages i-xxiv
    Download chapter PDF

  2. Quantitation of Proteins

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Protein Determination by UV Absorption

      • Alastair Aitken, Michèle P. Learmonth
      Pages 3-6

    3. The Lowry Method for Protein Quantitation

      • Jakob H. Waterborg
      Pages 7-9

    4. The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

      • John M. Walker
      Pages 11-14

    5. The Bradford Method for Protein Quantitation

      • Nicholas J. Kruger
      Pages 15-21

    6. Ultrafast Protein Determinations Using Microwave Enhancement

      • Robert E. Akins, Rocky S. Tuan
      Pages 23-30

    7. The Nitric Acid Method for Protein Estimation in Biological Samples

      • Scott A. Boerner, Yean Kit Lee, Scott H. Kaufmann, Keith C. Bible
      Pages 31-40

    8. Quantitation of Tryptophan in Proteins

      • Alastair Aitken, Michèle Learmonth
      Pages 41-44

    9. Flow Cytometric Quantitation of Cellular Proteins

      • Thomas D. Friedrich, F. Andrew Ray, Judith A. Laffin, John M. Lehman
      Pages 45-50

    10. Kinetic Silver Staining of Proteins

      • Douglas D. Root, Kuan Wang
      Pages 51-54

  3. Electrophoresis of Proteins and Peptides and Detection in Gels

    1. Front Matter

      Pages 55-55
      Download chapter PDF

    2. Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

      • John M. Walker
      Pages 57-60

    3. SDS Polyacrylamide Gel Electrophoresis of Proteins

      • John M. Walker
      Pages 61-67

    4. Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

      • John M. Walker
      Pages 69-72

    5. SDS-Polyacrylamide Gel Electrophoresis of Peptides

      • Ralph C. Judd
      Pages 73-79

    6. Identification of Nucleic Acid Binding Proteins Using Nondenaturing Sodium Decyl Sulfate Polyacrylamide Gel Electrophoresis (SDecS-PAGE)

      • Robert E. Akins, Rocky S. Tuan
      Pages 81-86

    7. Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins

      • Robert E. Akins, Rocky S. Tuan
      Pages 87-101

    8. Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins

      • Jakob H. Waterborg
      Pages 103-111

    9. Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones

      • Jakob H. Waterborg
      Pages 113-123
Back to top

Keywords

About this book

The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular Biology™ series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.

Reviews

"the breadth of techniques covered is large and this alone makes the book of practical value. . .The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory. . .It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins. . .This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory."-Doody's Health Sciences Book Review Journal

"The wide acceptance of this handbook in the former edition has resulted to this second thoroughly revised and extended second edition considering the recent significant developments in the field of protein chemistry. It is an excellent collection of methods and protocols, giving an essential and compact guide for those new as well as those well established to protein laboratory techniques." - Ernahrungforschung

Review of the first edition:
"The Protein Protocols Handbook has arrived at the right place at the right time."-Trends in Cell Biology

From Reviews of the First Edition
"...a must for researchers in protein chemistry and especially those with less experience..."
-Doody's Health Sciences Book Review Journal
"...a very useful tool for daily work.." -Nahrung-Food
"...definitely an indispensable benchtop handbook...highly recommended for all levels of research within the field." -Bioseparation
"...stands out in an already overcrowded field..."
-Aslib Book Guide

"In his preface the editor wishes the reader luck in his protein analysis, his book represents an invaluable compendium to help achieve this aim." - Molecular Biotechnology

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    John M. Walker

Bibliographic Information

  • Book Title: The Protein Protocols Handbook

  • Editors: John M. Walker

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1385/1592591698

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 2002

  • eBook ISBN: 978-1-59259-169-5Published: 02 October 2007

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 2

  • Number of Pages: XXIV, 1146

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation