Overview
- Editors:
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Myrtle A. Davis
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University of Maryland School of Medicine, Baltimore
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Table of contents (7 protocols)
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- Martin Poot, Robert H. Pierce, Terrance J. Kavanagh
Pages 11-36
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- Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski
Pages 37-58
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- Matthew A. Wallig, Curtis M. Chan, Nancy A. Gillett
Pages 59-76
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- Richard W. E. Clarkson, Catherine A. Boucher, Christine J. Watson
Pages 97-117
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- Calvin F. Roff, Amy M. Walz, Lisa B. Niehoff, David J. Sdano, Antoinette M. Bennaars, Jeffrey A. Cooper et al.
Pages 119-148
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Back Matter
Pages 149-156
About this book
Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.
Reviews
"The book emphasizes techniques that can be used to quantify apoptosis, such as cell morphology, flow cytometry, laser-scanning cytometry, ELISA, and DNA microarray. The flow cytometry and ELISA sections are the best, as they are applicable to a wide range of laboratories because of their ease of use do not require any extraordinary facilities. . .The book is well written and instructive, and the techniques described can be applied easily to topics in pharmacology and toxicology as well as in general apoptosis research. A strong point is that the authors emphasize the need to combine morphological assessment of the cell with other apoptotic parameters in order to fully characterize a cell as apoptotic."-Doody's Health Science Book Review Journal
Editors and Affiliations
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University of Maryland School of Medicine, Baltimore
Myrtle A. Davis
