Overview
- Editors:
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Joe O’Connell
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Department of Medicine, National University of Ireland, Cork, Ireland
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Table of contents (27 protocols)
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Highly Sensitive Detection and Analysis of mRNA
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- Susanne Greber-Platzer, Brigitte Balcz, Christine Fleischmann, Gert Lubec
Pages 29-58
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- Triona Goode, Wen-Zhe Ho, Terry O’Connor, Sandra Busteed, Steven D. Douglas, Fergus Shanahan et al.
Pages 65-79
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Quantitative RT-PCR
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- Joe O’Connell, Aileen Houston, Raymond Kelly, Darren O’Brien, Aideen Ryan, Michael W. Bennett et al.
Pages 93-102
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- Franz Bachmair, Christian G. Huber, Guenter Daxenbichler
Pages 103-116
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- Kaisa Nieminen, Markus Halminen, Matti Waris, Mika Mäkelä, Johannes Savolainen, Minna Sjöroos et al.
Pages 117-127
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- Jian-Ping Lai, Steven D. Douglas, Wen-Zhe Ho
Pages 129-147
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Detection and Analysis of RNA Viruses
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Front Matter
Pages 149-149
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- Brian Mullan, Liam J. Fanning, Fergus Shanahan, Daniel G. Sullivan
Pages 171-188
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In Situ Localization of mRNA Expression
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Front Matter
Pages 189-189
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About this book
Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
Reviews
"...RT-PCR succeeds in its goal to provide the reader with useful applications relevant to the "wider research community." -Clinical Chemistry
"It is clearly written and can be easily understood by the readers. It is recommended for students, geneticists, immunologists and other specialists in biomedicine." -Folia Microbiologica
Editors and Affiliations
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Department of Medicine, National University of Ireland, Cork, Ireland
Joe O’Connell
