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Ion Channel Localization

  • Book
  • © 2001

Overview

Editors:
  1. Anatoli N. Lopatin

    1. University of Michigan, Ann Arbor

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    You can also search for this editor in PubMed   Google Scholar

  2. Colin G. Nichols

    1. Washington University Medical School, St. Louis

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Pharmacology and Toxicology (MIPT)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Pharmacological Labeling of Ion Channels and Receptors

    1. Front Matter

      Pages 17-17
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    2. Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells

      • Thomas Budde
      Pages 1-15

    3. Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies

      • Daniel W. McPherson
      Pages 17-38

    4. GABAA Receptors

      • Cyrille Sur, John Atack
      Pages 39-56

    5. Localization of ATP P2X Receptors

      • Xuenong Bo, Geoffrey Burnstock
      Pages 57-73

    6. Small Conductance Calcium-Activated Potassium Channels in Rat Brain

      • Marc Borsotto
      Pages 75-91

    7. Mapping N-Type Calcium Channel Distributions with H-Conotoxins

      • Geula M. Bernstein, Owen T. Jones
      Pages 93-117

    8. Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development

      • Zhengshan Dai, H. Benjamin Peng
      Pages 119-143

    9. High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels

      • Lourival D. Possani, Baltazar Becerril, Jan Tytgat, Muriel Delepierre
      Pages 145-165

  3. Applications of Green Fluorescent Protein (GFP) Technology

    1. Front Matter

      Pages 166-166
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    2. Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells

      • Jan Eggermont, Dominique Trouet, Gunnar Buyse, Rudi Vennekens, Guy Droogmans, Bernd Nilius
      Pages 167-186

    3. Confocal Imaging of GFP-Labeled Ion Channels

      • Bryan D. Moyer, Bruce A. Stanton
      Pages 187-214

    4. Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes

      • Tooraj Mirshahi, Diomedes E. Logothetis, Massimo Sassaroli
      Pages 215-231

    5. Applications of Green Fluorescent Protein (GFP) Technology

      • Scott A. John, James N. Weiss
      Pages 233-248

    6. Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models

      • Joseph C. Koster, Colin G. Nichols
      Pages 249-260

    7. Mutant GFP- Based FRET Analysis of K+ Channel Organization

      • Elena N. Makhina, Colin G. Nichols
      Pages 261-274

    8. Delivering Ion Channels to Mammalian Cells by Membrane Fusion

      • David C. Johns, Uta C. Hoppe, Eduardo Marbán, Brian O’Rourke
      Pages 275-296

  4. Functional Assays in Ion Channel Localization

    1. Front Matter

      Pages 296-296
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    2. Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake

      • Cristóvão Albuquerque, Holly S. Engelman, C. Justin Lee, Amy B. MacDermott
      Pages 297-309
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About this book

Since the pioneering discoveries of Hodgkin, Huxley, and Katz, it has been clear that specific ion conductance pathways underlie electrical act- ity. Over the ensuing 50 years, there has been ever increasing, and occasi- ally explosive, changes in the scope of efforts to understand ion channel behavior. The introduction of patch clamp technology by Erwin Neher and Bert Sakmann about 20 years ago led to the realization of the great variety of novel ion channel species, and the subsequent revolution in cl- ing has revealed an even greater diversity of the underlying molecular entities. Today, advances in the study of ion channel structure and function c- tinue at a high pace, from angstrom resolution imaging of crystallized ch- nels to their genetic manipulations in animals. In this regard, the field is a balanced one that inquires not only what ion channel entities are there, or how they operate, but also where are these molecular electronic switches? However, this balance is not particularly well presented to the general sci- tific audience or to specialists in the field. There are plenty of wonderful and useful books and monographs, as well as conferences and meetings on v- tually every aspect of ion channel structure and function. However, we are unaware that the channel localization theme has been considered in a u- fied forum.

Reviews

"There is a nice balance of review and methodology, with an emphasis on methodological principles. The scope of the book provides a rich and comprehensive review of staid and state-of-the-art localization techniques and their rationales for use. This is a singular and needed addition to the field of ion channel physiology, cell biology and neurobiology"-Doody's Health Sciences Book Review Journal

"...both timely and will be of considerable interest to researchers of ion channel function....This book represents a collection of 24 methods of ion channel localization written by leading specialists and divided into four main parts. The first part concentrates on the fluorescent and radioligand binding of channel or receptors with specific pharmacological agents or toxins....Thus, the second part of this book concentrates on the quantification and cellular or subcellular visualization of ion channel expression in oocytes and immortal cell lines using green fluorescent proteins (GFP)....The third section of Ion Channel Localization: Methods and Protocols utilizes the functional properties of the channels for localization....One of the major achievements of Ion Channel Localization: Methods and Protocols in gathering so many of today's technologies of channel visualization is the candid and detailed descriptions the authors have provided of the advantages, pitfalls and 'tricks of the trade' of the methods under discussion. Descriptions are such I am confident that many scientists will be tempted to have a go themselves." - Clinical and Experimental Pharmacology and Physiology

Editors and Affiliations

  • University of Michigan, Ann Arbor

    Anatoli N. Lopatin

  • Washington University Medical School, St. Louis

    Colin G. Nichols

Bibliographic Information

  • Book Title: Ion Channel Localization

  • Editors: Anatoli N. Lopatin, Colin G. Nichols

  • Series Title: Methods in Pharmacology and Toxicology

  • DOI: https://doi.org/10.1385/1592591183

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-833-2Published: 01 April 2001

  • Softcover ISBN: 978-1-61737-211-7Published: 09 November 2010

  • eBook ISBN: 978-1-59259-118-3Published: 06 February 2008

  • Series ISSN: 1557-2153

  • Series E-ISSN: 1940-6053

  • Edition Number: 1

  • Number of Pages: XVII, 502

  • Topics: Pharmacology/Toxicology

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