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  • Book
  • © 2003

Capillary Electrophoresis of Carbohydrates

Editors:

  1. Pierre Thibault

    1. Caprion Pharmaceuticals, Montreal, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Susumu Honda

    1. Faculty of Pharmaceutical Sciences, Kinki University, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 213)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      PDF

  3. Sample Preparation Methods

    1. Front Matter

      Pages 25-25
      PDF

    2. Chemical and Enzymatic Release of Glycans from Glycoproteins

      • Tony Merry, Sviatlana Astrautsova
      Pages 27-40

    3. Derivatization of Carbohydrates

      • Shigeo Suzuki, John F. Kelly, Steven J. Locke, Pierre Thibault, Susumu Honda
      Pages 41-69

  4. Analysis of Mono- and Oligosaccharides

    1. Front Matter

      Pages 79-79
      PDF

    5. Affinity Capillary Electrophoresis of Oligosaccharides

      • Atsushi Taga, Susumu Honda
      Pages 121-130

    6. Characterization of Glycosaminoglycans by Capillary Electrophoresis

      • Robert J. Linhardt, Toshihiko Toida
      Pages 131-144

  5. Analysis of Glycoconjugates and the Component Monosaccharides

    1. Front Matter

      Pages 145-145
      PDF

    3. Separation of Protein Glycoforms by Capillary Electrophoresis

      • Myriam Taverna, Nguyet Thuy Tran, Danielle Ferrier
      Pages 163-196

    4. Separation of Glycoproteins by Capillary Isoelectric Focusing

      • Ira S. Krull, Sarah Kazmi, Huijuan Zhong, Ling Chen Santora
      Pages 197-218

    5. Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry

      • Kevin P. Bateman, John F. Kelly, Pierre Thibault, Louis Ramaley, Robert L. White
      Pages 219-239

    6. Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry

      • Pierre Thibault, Adèle Martin, Michel Gilbert, Warren W. Wakarchuk, James C. Richards
      Pages 241-259

  6. Specific Applications

    1. Front Matter

      Pages 261-261
      PDF
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About this book

Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods.
Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
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Keywords

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Reviews

"...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches." -Pharmaceutical Research

"...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field." -Biomedical Chromatography

"...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems." - Chromatographia

"...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis." - Biochemistry
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Editors and Affiliations

  • Caprion Pharmaceuticals, Montreal, Canada

    Pierre Thibault

  • Faculty of Pharmaceutical Sciences, Kinki University, Japan

    Susumu Honda

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Bibliographic Information

  • Book Title: Capillary Electrophoresis of Carbohydrates

  • Editors: Pierre Thibault, Susumu Honda

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592945

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-0-89603-826-4Published: 14 January 2003

  • Softcover ISBN: 978-1-61737-206-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-294-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 318

  • Topics: Biochemistry, general, Carbohydrate Chemistry

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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