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Immunocytochemical Methods and Protocols

  • Book
  • © 1999

Overview

Editors:
  1. Lorette C. Javois

    1. Catholic University of America, Washington

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Part of the book series: Methods in Molecular Biology (MIMB, volume 115)

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Table of contents (52 protocols)

  1. Front Matter

    Pages i-xii
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  3. Tissue Preparation for Light Microscopic Analyses

    1. Front Matter

      Pages 43-43
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    2. Overview of Cell Fixatives and Cell Membrane Permeants

      • Melissa A. Melan
      Pages 45-55

    3. Preparation of Frozen Sections for Analysis

      • Gary L. Bratthauer
      Pages 57-62

    4. Processing of Cytological Specimens

      • Gary L. Bratthauer
      Pages 63-70

    5. Processing of Tissue-Culture Cells

      • Gary L. Bratthauer
      Pages 71-76

    6. Processing of Tissue Specimens

      • Gary L. Bratthauer
      Pages 77-84

    7. Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections

      • Laszlo Krenacs, Tibor Krenacs, Mark Raffeld
      Pages 85-93

  4. Light Microscopic Detection Systems

    1. Front Matter

      Pages 95-95
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    2. Overview of Fluorochromes

      • J. Michael Mullins
      Pages 97-105

    3. Direct Immunofluorescent Labeling of Cells

      • Lorette C. Javois
      Pages 107-111

    4. Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells

      • Mark C. Willingham
      Pages 113-119
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Keywords

About this book

Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.

Reviews

"This second edition provides detailed protocols for immunocytochemical localization of tissue constituents. The book is well balanced and suited for basic and clinical research scientists. . .The editor has successfully compiled this book for the intended readers. The editor and contributors are credible. . .The protocol style format is useful for a first experiment. The illustrations are attractive and informative. The book as a whole is nicely done. . .I find this a very useful and practical addition to all fields of biology and medicine. It is very user friendly and should be a great reference for those designing experiments and writing."-Doody's Health Sciences Book Review Journal

"...this excellent book on Methods and Protocols can be recommended to both investigators starting out in the field of immunocytochemistry and to basic and clinical researchers already working in the field because it contains a plethora of methods and protocols for the demonstration of moleculesin many different normal and pathological situations and also under different experimental conditions." - Journal of Chemical Neuroanatomy

"...this book would make a valuable addition to laboratories currently involved in research using immunocytochemical techniques and would be particularly useful for those wishing to introduce these techniques into their research programmes...Given the depth of coverage of theory behind various techniques and the detailed notes that accompany each set of protocols, the book would also make an ideal teaching aid."-Molecular Biotechnology

Editors and Affiliations

  • Catholic University of America, Washington

    Lorette C. Javois

Bibliographic Information

  • Book Title: Immunocytochemical Methods and Protocols

  • Editors: Lorette C. Javois

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592139

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • eBook ISBN: 978-1-59259-213-5Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: 465

  • Topics: Biochemistry, general

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