Neural Transplantation Methods

1. Front Matter

   Pages i-xv

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2. Sources of Cells for Transplantation

   1. Front Matter

      Pages 1-1

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   2. Dissecting Embryonic Neural Tissues for Transplantation

      • Stephen B. Dunnett, Anders Björklund

      Pages 3-25

   3. Pregraft Tissue Storage Methods for Intracerebral Transplantation

      • Hansjörg Sauer

      Pages 27-40

   4. Neural Stem Cells for Transplantation

      • Siddharthan Chandran, Clive N. Svendsen

      Pages 41-54

   5. Immortalized Cells

      • Vincent M. Mann, John D. Sinden

      Pages 55-72

   6. Cells from Testis for Transplantation in the CNS

      • Alison E. Willing, Paul R. Sanberg

      Pages 73-88

   7. Engineered Cells and Ex Vivo Gene Transfer

      • Cecilia Lundberg

      Pages 89-102

   8. Intracerebral Gene Transfer Using Viral Vectors

      • Ronald J. Mandel, Steve Hardy, Richard O. Snyder, Luigi Naldini, Stuart E. Leff

      Pages 103-130

3. Methods of Implantation

   1. Front Matter

      Pages 131-131

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   2. Basic Transplantation Methods in Rodent Brain

      • Stephen B. Dunnett, Anders Björklund

      Pages 133-148

   3. Intraocular Grafting

      • Ingrid Strömberg

      Pages 149-167

   4. Neural Transplantation in the Developing CNS

      • Anthony J. Castro, Jens Zimmer

      Pages 169-194

   5. Preparation of Cell Suspensions for Transplantation

      • Roger A. Barker, Anne E. Rosser, Stephen B. Dunnett

      Pages 195-205

   6. Microtransplantation of Nigral Dopamine Neurons

      • Guido Nikkhah, Christian Winkler, Alexandra Rödter, Madjid Samii

      Pages 207-231

   7. Application of Polymer-Encapsulated Cell Therapy for CNS Diseases

      • Dwaine F. Emerich, Shelley R. Winn

      Pages 233-277

   8. Neural Transplantation in Parkinsonian Primates

      • Lucy E. Annett

      Pages 279-304

   9. Transplanting Myelinogenic Cells into the CNS

      • Robin J. M. Franklin, William F. Blakemore

      Pages 305-317

   10. Labeling and Identifying Grafted Cells

       • Alan R. Harvey

       Pages 319-361

4. Factors in Graft Survival and Function

   1. Front Matter

      Pages 363-363

      PDF

we might seek alternative sources of donor tissues. Genetic en- neering, expansion of precursor cells, generation of immortalized cell lines, and transplantation between species are all under active investigation. Although significant difficulties remain for each of these alternatives, the problems appear soluble and relevant knowledge is expanding rapidly. As we enter the twenty-first century, the place of neural tra- plantation in experimental neuroscience is continuing to evolve. Rather than being a topic in its own right, neural transplantation increasingly serves as just another technique in the researcher's armory--alongside lesions, pharmaceuticals, gene transfer, and a variety of other techniques--for the experimental manipulation of brain structure and function. This is particularly true for studies of degeneration, plasticity, regeneration, and recovery of function in the nervous system, topics of increasing importance as experimental neurobiology is required to serve the higher needs of neurological and mental health in aging societies. Within this evolving context, Neural Transplantation Methods seeks to serve a particular need: to provide experimental neu- scientists with a source book of information to enable them to select and adapt transplantation techniques to their own expe- mental programs. All authors have been asked to address prac- cal issues, to enable the reader to assess what is available, what are the alternatives, what are the practical issues to be resolved in applying a particular protocol and getting it to work reliably in their unique experimental context.

"...a book with so much information is very valuable for neuroscientists."- Clinical Neurology and Neurosurgery

"Neural Transplantation Methods seeks to serve a particular need: to provide experimental neuroscientists with a source book of information to enable them to select and adapt transplantation techniques to their own experimental programs....Neural Transplantation Methods is a very interesting, up-to-date, and complete book on the technical and methodological issues in relation with experimental neural transplantation. It is a perfect source book of information for those who want to include the transplantation strategy in their experimental program." - Acta Neurologica Belgica

• University of Cambridge, Cambridge, UK

  Stephen B. Dunnett

• Cardiff University, Cardiff, UK

  Stephen B. Dunnett

• University of Saskatchewan, Saskatoon, Canada

  Alan A. Boulton

• University of Alberta, Edmonton, Canada

  Glen B. Baker

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