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Hepatocellular Carcinoma

Methods and Protocols

  • Book
  • © 2000

Overview

Editors:
  1. Nagy A. Habib

    1. Liver Surgery Section, Imperial College School of Medicine, Hammersmith Hospital, London, UK

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 45)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
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  2. The Clinical Problem

    1. Front Matter

      Pages 1-34
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    2. Hepatocellular Carcinoma

      • Valery Usatoff, Nagy A. Habib
      Pages 3-20

    3. Medical Management of Hepatocellular Carcinoma

      • Stephen M. Riordan, Roger Williams
      Pages 21-34

  3. HCC Carcinogenesis

    1. Front Matter

      Pages 35-96
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    2. Possible Involvement of the NS3 Protein of Hepatitis C Virus in Hepatocarcinogenesis

      • Satoshi Ishido, Tsunenori Fujita, Hak Hotta
      Pages 37-55

    3. Abrogation of p53-Induced Apoptosis by the Hepatitis B Virus X Gene

      • Xin Wei Wang
      Pages 57-70

    4. Hepatocellular Carcinoma

      • Carmen Vandelli, Francesco Renzo
      Pages 71-96

  4. Molecular and Biological Characteristics

    1. Front Matter

      Pages 97-173
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    2. The Impact of Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV)

      • Maurizia Rossana Brunetto, Filippo Oliveri, Ferruccio Bonino
      Pages 99-112

    3. Mutation of p53 Tumor Suppressor Gene in Hepatocellular Carcinoma

      • Apollonia Tullo, Elisabetta Sbisà
      Pages 113-130

    4. Diagnostic Markers in Hepatocellular Carcinoma Using Immunohistochemical Techniques

      • Yaw Ohene-Abuakwa, Massimo Pignatelli
      Pages 131-138

    5. Assessing Matrix Metalloproteinase Expression and Activity in Hepatocellular Carcinomas

      • Orlando Musso, Bruno Clément, Nathalie Théret
      Pages 139-156

    6. Isolation of Novel Markers for Hepatocellular Carcinoma by a Subtraction-Enhanced Display Technique

      • Chuan-Ging Wu
      Pages 157-165

    7. Measurement of Protein Expression of p53, p21WAF1, and Rb in Patients with Surgically Treated Hepatocellular Carcinoma by Using Catalyzed Signal-Amplification System

      • Takuji Naka, Tetsuya Kaneko, Nobuaki Kaibara
      Pages 167-173

  5. HCC Gene Therapy

    1. Front Matter

      Pages 175-269
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    2. Gene Therapy Vectors Harboring AFP Regulatory Sequences

      • Shuichi Kaneko, Taiki Tamaoki
      Pages 177-187

    3. Advantages and Disadvantages of Multiple Different Methods of Adenoviral Vector Construction

      • Prem Seth, James Higginbotham
      Pages 189-198

    4. Electron Microscopic Assessment of Adenovirus-Mediated Transfer

      • Catherine E. Sarraf
      Pages 199-206

    5. p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression

      • Ragai R. Mitry, Michael D. Kelly, Jian Zhao, Satoko Negishi, Marc R. Mansour, Nagy A. Habib
      Pages 207-220
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About this book

Advances in molecular characterization and novel gene-isolation techniques have vigorously expanded our understanding of hepatocellular carcinoma (HCC), a form of liver cancer that affects one million people annually, and generated many new therapeutic possibilities. In Hepatocellular Carcinoma: Methods and Protocols, Nagy Habib and a team of basic and clinical researchers describe the wide variety of powerful new laboratory-based molecular methods currently being used for investigating and treating this disease. The book focuses on gene therapy approaches, including the use of such vectors as lipids, adenovirus, and baculovirus, and virus detection assessment using electron microscopy. It also provides preclinical and clinical data on the killing of cancer cells using tumor-suppressor genes, antisense compounds to growth factors, immunotherapy (remove gene), and virus-directed enzyme prodrug therapy. A perspective on future treatment of the failing liver is given, along with a clinical protocol for p53 gene therapy.
Hepatocellular Carcinoma: Methods and Protocols offers experimental and clinical investigators a rich source of both basic science and clinical information on today's optimal use of gene therapy to treat and manage patients suffering from hepatocellular carcinoma.

Reviews

"...The molecular techniques are presented in a very useful format...This is a unique book with tools presented that can be directly applied to bench, translational, and clinical research. The step-by-step protocols are comprehensive and easy to understand." -Doody's Health Science Book Review Journal

Editors and Affiliations

  • Liver Surgery Section, Imperial College School of Medicine, Hammersmith Hospital, London, UK

    Nagy A. Habib

Bibliographic Information

  • Book Title: Hepatocellular Carcinoma

  • Book Subtitle: Methods and Protocols

  • Editors: Nagy A. Habib

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592590799

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2000

  • Hardcover ISBN: 978-0-89603-785-4Published: 10 May 2000

  • Softcover ISBN: 978-1-4899-4169-5Published: 11 August 2013

  • eBook ISBN: 978-1-59259-079-7Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIII, 302

  • Topics: Oncology

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