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Antibiotic Resistance Methods and Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Stephen H. Gillespie

    1. Department of Medical Microbiology, Royal Free and University College Medical School, University College London, London, UK

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 48)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
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  2. Diagnosis of Resistance

    1. Front Matter

      Pages 1-1
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    2. Multiplex Polymerase Chain Reaction Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci

      • Robin Patel, Jim R. Uhl, Franklin R. Cockerill III
      Pages 3-11

    3. Drug Susceptibility of Mycobacterium tuberculosisThrough the Mycolic Acid Index

      • José M. Viader-Salvadó, Martha Guerrero-Olazarán, Elvira Garza-González, Rolando Tijerina-Menchaca
      Pages 13-19

    4. Micro-Well Phage Replication Assay for Screening Mycobacteria for Resistance to Rifampin and Streptomycin

      • Ruth McNerney
      Pages 21-30

    5. Application of SSCP to Identification of Resistance Mutations

      • Timothy D. McHugh
      Pages 31-37

    6. Quantitative, Single-Tube, Nested, PCR (QSTN-PCR) for Determining the Antibiotic Susceptibility of Mycobacterium tuberculosis

      • Nainn-Tsyr Jou, Michael R. Liebling
      Pages 39-54

    7. Rapid Rifamycin Susceptibility Testing of Small-Volume Mycrobacterium tuberculosis Cultures by Detection of Precursor rRNA

      • William Brabant H., Gerard Cangelosi A.
      Pages 55-63

    8. Detection of Penicillin Resistance in Streptococcus pneumoniae by a Seminested PCR Strategy

      • Mignon du Plessis, Anthony M. Smith, Keith P. Klugman
      Pages 65-75

    9. Diagnosis of Penicillin Resistance by PCR-RFLP

      • Gail C. Whiting
      Pages 77-84

    10. Detection Methods of Glycopeptide-Resistant Staphylococcus aureus I

      • Hedeaki Hanaki, Keiichi Hiramatsu
      Pages 85-91

    11. Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II

      • Hedeaki Hanaki, Keiichi Hiramatsu
      Pages 93-101

    12. Multiplex PCR for the Rapid Simultaneous Speciation and Detection of Methicillin-Resistance and Genes Encoding Toxin Production in Staphylococcus aureus

      • Mark E. Jones, Karl Köhrer, Franz-Josef Schmitz
      Pages 103-112

    13. Chromogenic Detection of Aminoglycoside Phosphotransferases

      • Ana M. Amoroso, Gabriel O. Gutkind
      Pages 113-117

  4. Molecular Epidemiology

    1. Front Matter

      Pages 157-157
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    2. BOX PCR Fingerprinting for Molecular Typing of Streptococcus pneumoniae

      • Alex van Belkum, Peter W. M. Hermans
      Pages 159-168
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Keywords

About this book

At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls.
Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.

Reviews

"...an excellent, well-produced book, full of protocols on a range of modern molecular techniques, applicable not only to research on antibiotics resistance, but to general microbiology....The articles are of a uniformly high standard, well illustrated and referenced, which act as logical easy to follow protocols. this book is a must for anyone involved in research on antibiotic resistance; equally all microbiologists who use modern molecular methods should consult it in the expectation of finding well-described established techniques and numerous cutting-edge developments." - MicroBiology Today

Editors and Affiliations

  • Department of Medical Microbiology, Royal Free and University College Medical School, University College London, London, UK

    Stephen H. Gillespie

Bibliographic Information

  • Book Title: Antibiotic Resistance Methods and Protocols

  • Editors: Stephen H. Gillespie

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592590772

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2001

  • eBook ISBN: 978-1-59259-077-3Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XV, 287

  • Topics: Infectious Diseases

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