Colorectal Cancer

1. Front Matter

   Pages i-xiii

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2. Microdissection of Histologic Sections

   • Christopher A. Moskaluk

   Pages 1-13

3. Isolation of a Purified Epithelial Cell Population from Human Colon

   • James K. Roche

   Pages 15-20

4. Xenografting Human Colon Cancers

   • Jeffrey C. Harper, Reid B. Adams, Steven M. Powell

   Pages 21-24

5. Comparative Genomic Hybridization Technique

   • Wa′el El-Rifai, Sakari Knuutila

   Pages 25-33

6. Fluorescence In Situ Hybridization

   • Svetlana D. Pack, Zhengping Zhuang

   Pages 35-50

7. Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer

   • Jin Jen

   Pages 51-58

8. The SURF Technique

   • Darryl Shibata

   Pages 59-67

9. Microsatellite Instability Testing

   • Yann R. Parc, Kevin C. Halling

   Pages 69-79

10. Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer

    • Kevin C. Halling, Patrick C. Roche

    Pages 81-86

11. Mutation Detection in Colorectal Cancers

    • Julie M. Cunningham, David J. Tester, Stephen N. Thibodeau

    Pages 87-98

12. Mutation Detection inTGF-β Receptors

    • William M. Grady, Lois L. Myeroff, Hongmei He, Sanford Markowitz

    Pages 99-111

13. Direct Analysis for Familial Adenomatous Polyposis Mutations

    • Steven M. Powell

    Pages 113-128

14. I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer

    • Perry S. Chan, Steven L. Gersen

    Pages 129-135

15. Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas

    • Rhonda F. Souza, Stephen J. Meltzer

    Pages 137-148

16. Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors

    • Sam Thiagalingam

    Pages 149-165

17. Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations

    • Lauri A. Aaltonen, Stina Roth

    Pages 167-174

18. 17 Direct Sequencing for Peutz-Jeghers Gene LKB1 (STK11) Mutations

    • Lauri A. Aaltonen, Egle Avizienyte

    Pages 175-183

19. 18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations

    • Lauri A. Aaltonen, Stina Roth, Charis Eng

    Pages 185-193

20. Genetic Analysis Using Microarrays

    • Wa’el El-Rifai, Sakari Knuutila

    Pages 195-203

The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench. Of note, most of the chapters in this volume are written by those scientists who p- neered these methods and assays in their respective fields. The chapters in Colorectal Cancer: Methods and Protocols describe “state of the art” methods to analyze colorectal tumors, ranging from gross mic- dissection of specimens to specific molecular analyses. Included are coverages of mutational assays, instability testing, immunohistochemical assays, chro- somal studies, and gene expression analyses. The goal of our volume is to facilitate the performance of colorectal tumor biological experiments by investigators at various levels of training—from graduate students and postdoctoral fellows to principal investigators who desire to advance our understanding of colon cancer development.

"This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum

"...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy

• University of Virginia Health System, Charlottesville

  Steven M.

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