Atherosclerosis

1. Front Matter

   Pages i-xi

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2. Animal Models of Diet-Induced Atherosclerosis

   • Angela F. Drew

   Pages 1-6

3. Mechanical Injury Models

   • Rodney J. Dilley

   Pages 7-13

4. Genetically Manipulated Models of Atherosclerosis in Mice

   • Qing Xiao

   Pages 15-26

5. Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation

   • Kishor M. Wasan, Shawn M. Cassidy, Allison L. Kennedy, Kathy D. Peteherych

   Pages 27-35

6. Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol

   • Joan A. Higgins, John M. Graham, Ian G. Davies

   Pages 37-49

7. Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of Iodixanol

   • John M. Graham, Bruce A. Griffin, Ian G. Davies, Joan A. Higgins

   Pages 51-59

8. 7 Detecting Cholesteryl Ester Transfer Protein in Plasma

   • Andreas Ritsch, Josef R. Patsch

   Pages 61-76

9. 8 Determination of Plasma Homocysteine

   • Roberto Accinni, Oberdan Parodi

   Pages 77-103

10. 9 Assay for Serum Glycated Lipoproteins

    • Akira Tanaka

    Pages 105-111

11. 10 Lipoprotein(a) Quantitation

    • Michael Bender, Werner Gross

    Pages 113-122

12. Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels

    • Eleni Papakonstantinou, Michael Roth, George Karakiulakis

    Pages 123-136

13. Culture of Human Smooth Muscle Cells

    • Marisa A. Gallicchio

    Pages 137-146

14. Culture of Human Endothelial Cells

    • Marisa A. Gallicchio

    Pages 147-161

15. Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells

    • George Karakiulakis, Eleni Papakonstantinou, Michael Roth

    Pages 163-169

16. Collection and Processing of Arterial Specimens for Histological Analysis

    • Angela F. Drew

    Pages 171-175

17. Intravascular Ultrasound Imaging in the Quantitation of Atherosclerosis in Vivo

    • Hannu I. Manninen

    Pages 177-186

18. Identification of Cell Types and Quantification of Lesion Composition

    • Rodney J. Dilley

    Pages 187-194

19. 1Nonradioactive In Situ Hybridization in Atherosclerotic Tissue

    • Jim Apostolopoulos

    Pages 195-206

20. Evaluation of Angiogenesis

    • Marcy Silver, Jeffrey M. Isner

    Pages 207-211

Atherosclerosis: Experimental Methods and Protocols aims to provide the reader with a compilation of techniques that will prove useful to active investigators across the field of experimental atherosclerosis research. In fact, this volume is unique, the first devoted to a broad spectrum of techniques and assays, some adopted from other disciplines, not previously brought together in one book. Our approach is designed to permit researchers to select the techniques that will answer their particular sets of questions, in any of the expanding number of both animal models and in vitro systems now available for studying factors contributing to the development or progression of athe- sclerotic lesions. Researchers can only benefit from this collection of relevant techniques, written and explained by experts in each of these fields. Both investigators beginning in the field of atherosclerosis studies and researchers entering the field from related but different areas of study will benefit from Atherosclerosis: Experimental Methods and Protocols. Sufficient background is provided for a beginner to carry out the techniques described in the chapters, yet great depth is achieved owing to the special expertise of the authors. Researchers new to the field of atherosclerosis will appreciate the benefits of having these techniques gathered in one volume for their inves- gations. In addition, researchers already in the field of atherosclerosis research may benefit from the wide array of techniques and ideas provided by enjoying expanded opportunities to investigate their hypotheses.

"Methods for setting up animal model periments and special assays of blood and cellular moments of the pathologic processes involved are thoroughly described and referenced. Newly emerging animal models based on genetic manipulation are described in depth, with sufficient detail to broaden the scope of research by seasoned investigators to permit the novice to enter this field. ...Anyone working in vascular pathology and atherosclerosis research will find a wealth of practical information describing modern research methods." - Clinical and Applied Thrombosis/Hemostasis

"Atherosclerosis is recommended for academic health science libraries. It is also recommended to individual laboratories that conduct research in the area of atherosclerosis or heart disease." -E-STREAMS

• Children’s Hospital Research Foundation, Cincinnati

  Angela F. Drew

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