NMDA Receptor Protocols

1. Front Matter

   Pages i-x

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2. Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes

   • Fumio Nakamura, Yoshio Goshima, Stephen M. Strittmatter, Susumu Kawamoto

   Pages 1-18

3. Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors

   • Susumu Kawamoto, Satoshi Hattori, Shigeo Uchino, Masayoshi Mishina, Kenji Okuda

   Pages 19-32

4. Transient Expression of Functional NMDA Receptors in Mammalian Cells

   • Paul L. Chazot, Miroslav Cik, F. Anne Stephenson

   Pages 33-42

5. Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells

   • Mark A. Varney, Fen-Fen Lin, Christine Jachec, Sara P. Rao, Stephen D. Hess, Edwin C. Johnson et al.

   Pages 43-59

6. Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells

   • Erik A. Whitehorn, William J. Dower, Min Li

   Pages 61-72

7. Immunocytochemistry of NMDA Receptors

   • Ronald S. Petralia, Robert J. Wenthold

   Pages 73-92

8. The Use of Ligand Binding in Assays of NMDA Receptor Function

   • Ian J. Reynolds, Terre A. Sharma

   Pages 93-102

9. Calmodulin Modification of NMDA Receptors

   • Su Zhang, Richard L. Huganir

   Pages 103-111

10. Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors

    • Robert J. Wenthold, Jaroslav Blahos II, Kyung-Hye Huh, Ronald S. Petralia

    Pages 113-119

11. Redox Sensitivity of NMDA Receptors

    • Stuart A. Lipton

    Pages 121-130

12. In Vitro Selection of Peptides Acting on NMDA Receptors

    • Steven E. Cwirla, William J. Dower, Min Li

    Pages 131-141

13. NMDA Receptor Pharmacology and Analysis of Patch-Clamp Recordings

    • Jerry M. Wright, Robert W. Peoples

    Pages 143-153

14. Determination of Membrane Topology of Glutamate Receptors

    • Hendrika M. A. VanDongen, Antonius M. J. VanDongen

    Pages 155-166

15. Engineering the NMDA Receptor Channel Lining

    • Antonio V. Ferrer-Montiel, Mauricio Montal

    Pages 167-178

16. High-Throughput Functional Detection of NMDA Receptor Activity

    • Martin Donal Cunningham, Sara Mary Cunningham

    Pages 179-188

17. Back Matter

    Pages 189-196

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Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.

"...In this volume of Methods in Molecular Biology great care has been taken to present the most modern approaches to solving the mysteries of the NMDA receptor...Each chapter includes detailed protocols and highly useful 'notes' that explain possible problems, sources of technical artifacts and how to overcome them....this book is informative and practical and would serve any channel or receptor researcher well." -Molecular Biotechnology

• Johns Hopkins School of Medicine, Baltimore

  Min Li

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