Overview
- Editors:
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Catherine H. Schein
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University of Texas Medical Branch, Galveston
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Table of contents (32 protocols)
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Front Matter
Pages i-xvii
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Characterizing Nuclease Activity
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- M. Victòria Nogués, Claudi M. Cuchillo
Pages 15-24
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- Tania N. Gonzalez, Peter Walter
Pages 25-36
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- Gregor Meiss, Oleg Gimadutdinow, Peter Friedhoff, Alfred M. Pingoud
Pages 37-48
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- Brian Beecher, Bruce A. McClure
Pages 65-85
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- Francesco Acquati, Cinzia Nucci, Marco G. Bianchi, Tatiana Gorletta, Roberto Taramelli
Pages 87-101
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Inhibitors and Activators of Nucleases
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Front Matter
Pages 103-103
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- Zeger Debyser, Peter Cherepanov, Wim Pluymers, Erik De Clercq
Pages 139-155
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- Richard V. Giles, David M. Tidd
Pages 157-182
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- Catherine Bisbal, Tamim Salehzada, Michelle Silhol, Camille Martinand, Florence Le Roy, Bernard Lebleu
Pages 183-198
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Relating Nucleas Structure and Function
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Front Matter
Pages 199-199
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- Gennaro Marino, Piero Pucci, Margherita Ruoppolo
Pages 227-236
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- Andres Ramos, Annalisa Pastore
Pages 237-248
About this book
Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical relevance. Part III, “Relating Nuclease Structure and Function,” provides an overview of methods to determine or model the 3-D structure of nucleases and their complexes with substrates and inhibitors. A 3-D structure can greatly aid the rational design of nucleases and inhibitors for specific purposes. Part IV, “Nucleases in the Clinic,” summarizes assays and protocols suitable for use with t- sues and for nuclease based therapeutics.
Editors and Affiliations
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University of Texas Medical Branch, Galveston
Catherine H. Schein
