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Posttranslational Modification of Proteins

Tools for Functional Proteomics

  • Book
  • © 2002

Overview

Editors:
  1. Christoph Kannicht

    1. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Berlin, Germany

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 194)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Assignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry

    • Jiang Wu, J. Throck Watson
    Pages 1-22

  3. Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods

    • George N. Saddic, Mary Beth Ebert, Shirish T. Dhume, Kalyan R. Anumula
    Pages 23-36

  4. Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB)

    • Rolf Nuck
    Pages 37-44

  5. Separation of N-Glycans by HPLC

    • Martin Gohlke
    Pages 45-61

  6. Enzymatic Sequence Analysis of N-Glycans

    • Christoph Kannicht, Anke Flechner
    Pages 63-72

  7. Immunological Detection of O-GlcNAc

    • Monika Rex-Mathes, Jürgen Koch, Sabine Werner, Lee S. Griffith, Brigitte Schmitz
    Pages 73-87

  8. Analysis of O-Glycosylation

    • Juan J. Calvete, Libia Sanz
    Pages 89-100

  9. Characterization of Site-Specific Glycosylation

    • Katalin F. Medzihradszky
    Pages 101-125

  10. Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid

    • Shirish T. Dhume, Mary Beth Ebert, George N. Saddic, Kalyan R. Anumula
    Pages 127-142

  11. Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa

    • Peter Gerold, Ralph T. Schwarz
    Pages 143-158

  12. Analysis of S-Acylation of Proteins

    • Michael Veit, Evgeni Ponimaskin, Michael F. G. Schmidt
    Pages 159-178

  13. Immunoblotting Methods for the Study of Protein Ubiquitination

    • Edward G. Mimnaugh, Leonard M. Neckers
    Pages 179-203

  14. Analysis of Methylation and Acetylation in E. coli Ribosomal Proteins

    • Randy J. Arnold, James P. Reilly
    Pages 205-210

  15. Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry

    • Jun Qin, Xiaolong Zhang
    Pages 211-221

  16. Analysis of Tyrosine-O-Sulfation

    • Jens R. Bundgaard, Anders H. Johnsen, Jens F. Rehfeld
    Pages 223-239

  17. α-Amidated Peptides: Approaches for Analysis

    • Gregory P. Mueller, William J. Driscoll
    Pages 241-257

  18. γ-Glutamate and p-Hydroxyaspartate in Proteins

    • Francis J. Castellino, Victoria A. Ploplis, Li Zhang
    Pages 259-268

  19. Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides

    • Verne Schirch, Sonia Delle Fratte, Martino di Salvo
    Pages 269-276

  20. Lysine Hydroxylation and Crosslinking of Collagen

    • Mitsuo Yamauchi, Masashi Shiiba
    Pages 277-290
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About this book

The majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation.
Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.

Reviews

"As indicated by the editor, this book will be useful to researchers involved in elucidating protein structure and function as well as those working to design proteins with specific function...this book offers detailed analytical protocols for identifying and characterizing most types of co- and post- translational modifications" - Pharmaceutical Research

"This book provides researchers in the biological sciences with the most updated methods to investigate co- and/or post-translational modifications of proteins...Either trained professionals and students working in the field of proteomics can take benefit for their research." - Proteomics

"It will definitely find appreciative readers among a wide audience of glycobiologists, biochemists, molecular biologists, biotechnologists, and researchers specializing in pharmacology and molecular medicine. This book will be a very useful tool for teachers and students of universities and institutes." -Biochemistry

Editors and Affiliations

  • Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Berlin, Germany

    Christoph Kannicht

Bibliographic Information

  • Book Title: Posttranslational Modification of Proteins

  • Book Subtitle: Tools for Functional Proteomics

  • Editors: Christoph Kannicht

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591817

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2002

  • Hardcover ISBN: 978-0-89603-678-9Published: 16 April 2002

  • Softcover ISBN: 978-1-4899-3864-0Published: 20 September 2013

  • eBook ISBN: 978-1-59259-181-7Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 322

  • Number of Illustrations: 19 b/w illustrations

  • Topics: Cell Biology

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