Overview
- Editors:
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M. A. Nasser Hajibagheri
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Imperial Cancer Research Fund, London, UK
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Table of contents (15 protocols)
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- J. Robin Harris, Marc Adrian
Pages 31-48
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- Rick T. Giberson, Richard S. Demaree Jr.
Pages 145-158
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- Nobukazu Araki, Tanenori Hatae
Pages 159-165
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- Jean-Guy Fournier, Françoise Escaig-Haye
Pages 167-181
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- M.A. Nasser Hajibagheri, Kenneth Sawin, Steve Gschmeissner, Ken Blight, Carol Upton
Pages 183-207
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- Maria Schnos, Ross B. Inman
Pages 229-243
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- A. John Morgan, Carole Winters, Stephen Stürzenbaum
Pages 245-276
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Back Matter
Pages 277-283
About this book
Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Reviews
"Chapter after chapter, methods are described in detail following a step-by-step schedule giving sufficient details to lead the researcher to precise and reproducible work. . .One of the great features of this book is a series of notes concluding every chapter. These notes pool a lot of important recommendations. If I can give a piece of good advice, just for these notes, buy this book and read all this valuable information."-Biochemie
"This ring-bound volume provides the reader with a useful source of comprehensive and accurate protocols for electron microscopic specimen preparation with an emphasis on the subcellular level..."-Microbiology Today
Editors and Affiliations
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Imperial Cancer Research Fund, London, UK
M. A. Nasser Hajibagheri
