Overview
- Editors:
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Bimal D. M. Theophilus
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Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK
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Ralph Rapley
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Department of Biosciences, University of Hertfordshire, Hatfield, UK
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Table of contents (19 protocols)
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- Ralph Rapley, Bimal D. M. Theophilus
Pages 13-15
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- Ralph Rapley, Ian J. Williams
Pages 23-27
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- Peter A. Davies, George Gray
Pages 51-55
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- Anu Wartiovaara, Ann-Christine Syvänen
Pages 57-63
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- Sara A. Dyer, Elaine K. Green
Pages 73-86
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- Naushin H. Waseem, Richard Bagnall, Peter M. Green, Francesco Giannelli
Pages 109-124
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- Ian J. Williams, Anne C. Goodeve
Pages 137-150
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- Laura Heisler, Chao-Hung Lee
Pages 165-178
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- Christian Jurinke, Dirk van den Boom, Charles R. Cantor, Hubert Köster
Pages 179-192
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About this book
1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
Reviews
"The book provides access to the highly productive and readily reproducible PCR-based mutation detection methods, that are behind many of today's major scientific and medical advances." - Clinical Laboratory International
Editors and Affiliations
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Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK
Bimal D. M. Theophilus
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Department of Biosciences, University of Hertfordshire, Hatfield, UK
Ralph Rapley