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Adenovirus Methods and Protocols

  • Book
  • © 1999

Overview

Editors:
  1. William S. M. Wold

    1. St. Louis University Health Science Center, St. Louis

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 21)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Preparation and Titration of CsCl-Banded Adenovirus Stock

    • Ann E. Tollefson, Terry W. Hermiston, William S. M. Wold
    Pages 1-9

  3. Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units

    • Terry W. Hermiston, Ann E. Tollefson, William S. M. Wold
    Pages 11-24

  4. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants

    • Gary Ketner, Julie Boyer
    Pages 25-32

  5. Manipulation of Early Region 4

    • Julie Boyer, Gary Ketner
    Pages 33-46

  6. Adenovirus DNA Packaging

    • Susanne I. Schmid, Patrick Hearing
    Pages 47-59

  7. Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome

    • C. S. H. Young, Andrea L. Nicolásvv, Heng Lu, Patricia L. Munz
    Pages 61-83

  8. Construction of Mouse Adenovirus Type 1 Mutants

    • Angela N. Cauthen, Katherine R. Spindler
    Pages 85-103

  9. Generation of Adenovirus-Specific Cytotoxic T Lymphocytes

    • Tim E. Sparer, Linda R. Gooding
    Pages 105-111

  10. Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells

    • Penelope J. Duerksen-Hughes, Linda R. Gooding
    Pages 113-120

  11. Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells

    • Joanna L. Shisler, Linda R. Gooding
    Pages 121-128

  12. Role of Natural Killer (NK) Cells in Immunity to Adenoviruses

    • John M. Routes
    Pages 129-141

  13. Determination of the Transforming Activities of Adenovirus Oncogenes

    • T. Subramanian, G. Chinnadurai
    Pages 143-149

  14. Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members

    • T. Subramanian, G. Chinnadurai
    Pages 151-156

  15. In Vitro Transcription

    • Paul M. Loewenstein, Chao-Zhong Song, Maurice Green
    Pages 157-168

  16. Cell Microinjection

    • Maurice Green, Andrew Thorburn, Paul M. Loewenstein
    Pages 169-193

  17. Immunoprecipitation of E1 A-Containing Protein Complexes

    • Elizabeth Moran, Peter Yaciuk
    Pages 195-202

  18. Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-lnfected Cells

    • Oliver Mühlemann, Göran Akusjärvia
    Pages 203-216

  19. Adenovirus Entry into Cells

    • Urs F. Greber, Michel Y. Nakano, Maarit Suomalainen
    Pages 217-230

  20. Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining

    • Eileen Bridge
    Pages 231-240
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About this book

In Adenovirus Methods and Protocols, William S.M. Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion, as well as tried and true step-by-step instructions.

Adenovirus Methods and Protocols will be useful to both entry-level and senior scientists seeking to enter the adenovirus field, to researchers from other areas wishing to construct adenovirus vectors for their own research, and to adenovirologists wanting to enter new sectors of research. Its cutting-edge techniques are certain to make it today's reference of choice, one from which even expert researchers will learn many productive and time-saving techniques.




Reviews

"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today

". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters

Editors and Affiliations

  • St. Louis University Health Science Center, St. Louis

    William S. M. Wold

Bibliographic Information

  • Book Title: Adenovirus Methods and Protocols

  • Editors: William S. M. Wold

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/0896035514

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1999

  • eBook ISBN: 978-1-59259-603-4Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIII, 352

  • Number of Illustrations: 72 b/w illustrations

  • Topics: Cell Biology, Human Genetics

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