Plant Cell Culture Protocols

1. Front Matter

   Pages i-xiv

   PDF

2. Introduction

   1. Front Matter

      Pages N1-N1

      PDF

   2. An Introduction to Plant-Cell Culture

      • Robert D. Hall

      Pages 1-18

3. Cell Culture and Plant Regeneration

   1. Front Matter

      Pages N3-N3

      PDF

   2. Callus Initiation, Maintenance, and Shoot Induction in Rice

      • Nigel W. Blackhall, Joan P. Jotham, Kasimalal Azhakanandam, J. Brian Power, Kenneth C. Lowe, Edward C. Cocking et al.

      Pages 19-29

   3. Callus Initiation, Maintenance, and Shoot Induction in Potato

      • Rosario F. Curry, Alan C. Cassells

      Pages 31-42

   4. Somatic Embryogenesis in Barley Suspension Cultures

      • Makoto Kihara, Hideyuki Funatsuki, Kazutoshi Ito, Paul A. Lazzeri

      Pages 43-49

   5. Somatic Embryogenesis in Picea Suspension Cultures

      • Ulrika Egertsdotter

      Pages 51-60

   6. Specialized Techniques

      1. Direct, Cyclic Somatic Embryogenesis of Cassava for Mass Production Purposes

         • Krit J. J. M. Raemakers, Evert Jacobsen, Richard G. F. Visser

         Pages 61-70

      2. Immature Inflorescence Culture of Cereals

         • Sonriza Rasco-Gaunt, Pilar Barcelo

         Pages 71-81

      3. Cryopreservation of Rice Tissue Cultures

         • Erica E. Benson, Paul T. Lynch

         Pages 83-94

      4. Noncryogenic, Long-Term Germplasm Storage

         • Ali Golmirzaie, Judith Toledo

         Pages 95-101

4. Plant Propagation In Vitro

   1. Front Matter

      Pages N5-N5

      PDF

   2. Micropropagation of Strawberry via Axillary Shoot Proliferation

      • Philippe Boxus

      Pages 103-114

   3. Meristem-Tip Culture for Propagation and Virus Elimination

      • Brian W. W. Grout

      Pages 115-125

   4. Specialized Techniques

      1. Clonal Propagation of Orchids

         • Brent Tisserat, Daniel Jones

         Pages 127-134

      2. In Vitro Propagation of Succulent Plants

         • Jill Gratton, Michael F. Fay

         Pages 135-140

      3. Micropropagation of Flower Bulbs

         • Merel M. Langens-Gerrits, Geert-Jan M. De Klerk

         Pages 141-147

      4. Clonal Propagation of Woody Species

         • Indra S. Harry, Trevor A. Thorpe

         Pages 149-157

      5. Spore-Derived Axenic Cultures of Ferns as a Method of Propagation

         • Matthew V. Ford, Michael F. Fay

         Pages 159-168

Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

". . . a valuable update on the technology, with emphasis on methods for micropropagation and gene transfer. . .the editor has assembled an excellent range of expert authors and the volume is well balanced and up-to-date. . .thoroughly recommended."-Plant Growth Regulation

"Protocols are presented for many agronomically important plants including the culture and regeneration of rice, potato, barley, casava, spruce, Pasiflora, Lolium, and various species of Brassica. The organization of all of the chapters is consistent including an introduction, a materials section divided into appropriate subsections, a similarly subdivided methods section, a notes section that contains important observations to make or precautions to take, and a final reference section. . .the techniques are well written and include specific examples and step-by-step methods with appropriate references. . .will make an excellent addition to the library of researchers who wish to extend their collection of basic plant cell culture techniques."-Quarterly Review of Biology

"The combination of many years of experience with new experimental entries is often very successful and of high quality...represents one of the highest quality manuals on this topic published with the last ten years...it will assure the relevance of an important scientific discipline, plant biotechnology, in today's world."-Biologia Plantarum

"The book brings together a series of well written, step-by-step protocols on the most frequently used techniques in plant cell and tissue culture, covering various aspects of culture initiation, maintenance, long-term storage and manipulation....In addition to protocols on widely applied methods, each part includes representative protocols for more specialized techniques....All the chapters are well structured, profusely illustrated with original photographs and line drawings, and contain detailed descriptions of materials, culture media and comprehensivelydescribed experimental procedures. The reader will certainly appreciate the precautionary notes and additional hints on each technique and each protocol provided at the end of each chapter."-Elsevier

"This book presents a comprehensive collection of applicable techniques for plant cell and tissue culture. The methods cover culture initiation, maintenance, manipulation, application and long-term storage, with emphasis on techniques for genetic modification and micropropagation." - Lavoisier Librairie

• CPRO-DLO, Wageningen, The Netherlands

  Robert D. Hall

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