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Confocal Microscopy

Methods and Protocols

  • Book
  • © 1999

Overview

Editors:
  1. Stephen W. Paddock

    1. Department of Molecular Biology, University of Wisconsin, Madison

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Part of the book series: Methods in Molecular Biology (MIMB, volume 122)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. An Introduction to Confocal Imaging

    • Stephen W. Paddock
    Pages 1-34

  3. Practical Considerations for Collecting Confocal Images

    • David Carter
    Pages 35-57

  4. Fluorescent Probes for Confocal Microscopy

    • Christopher Cullander
    Pages 59-73

  5. Imaging Gene Expression Using Antibody Probes

    • Nadean L. Brown
    Pages 75-91

  6. Single and Double FISH Protocols for Drosophila

    • Sarah C. Hughes, Henry M. Krause
    Pages 93-101

  7. Confocal Microscopy of Plant Cells

    • Carol L. Wymer, Alison F. Beven, Kurt Boudonck, Clive W. Lloyd
    Pages 103-130

  8. Preparation of Yeast Cells for Confocal Microscopy

    • Audrey L. Atkin
    Pages 131-139

  9. Confocal Methods for Caenorhabditis elegans

    • Sarah L. Crittenden, Judith Kimble
    Pages 141-151

  10. Imaging Sea Urchin Fertilization

    • Jon M. Holy
    Pages 153-166

  11. Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy

    • James A. Langeland
    Pages 167-172

  12. Confocal Microscopy on Xenopus laevis Oocytes and Embryos

    • Denise L. Robb, Chris Wylie
    Pages 173-183

  13. Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos

    • Mark S. Cooper, Leonard A. D’Amico, Clarissa A. Henry
    Pages 185-204

  14. Confocal Imaging of Living Cells in Intact Embryos

    • Paul M. Kulesa, Scott E. Fraser
    Pages 205-222

  15. Live Confocal Analysis with Fluorescently Labeled Proteins

    • Helen Francis-Lang, Jonathan Minden, William Sullivan, Karen Oegema
    Pages 223-239

  16. Live Imaging with Green Fluorescent Protein

    • Jim Haseloff, Emma-Louise Dormand, Andrea H. Brand
    Pages 241-259

  17. Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging

    • Donald M. O’Malley, Barry J. Burbach, Paul R. Adams
    Pages 261-303

  18. Intracellular pH and pCa Measurement

    • Michal Opas, Ewa Dziak
    Pages 305-313

  19. Measuring Dynamic Cell Volume In Situ by Confocal Microscopy

    • Rachel J. Errington, Nick S. White
    Pages 315-340

  20. Imaging Thick Tissues with Confocal Microscopy

    • Delphine Imbert, Janet Hoogstraate, Emmeline Marttin, Christopher Cullander
    Pages 341-355
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About this book

In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish.

Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.





Reviews

"This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology

"...almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely...The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International

" This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes...there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today

"...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations..."-Ultramicroscopy

"...excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology

Editors and Affiliations

  • Department of Molecular Biology, University of Wisconsin, Madison

    Stephen W. Paddock

Bibliographic Information

  • Book Title: Confocal Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: Stephen W. Paddock

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259722X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • Softcover ISBN: 978-1-61737-062-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-722-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 446

  • Topics: Cell Biology

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