Confocal Microscopy

1. Front Matter

   Pages i-xii

   PDF

2. An Introduction to Confocal Imaging

   • Stephen W. Paddock

   Pages 1-34

3. Practical Considerations for Collecting Confocal Images

   • David Carter

   Pages 35-57

4. Fluorescent Probes for Confocal Microscopy

   • Christopher Cullander

   Pages 59-73

5. Imaging Gene Expression Using Antibody Probes

   • Nadean L. Brown

   Pages 75-91

6. Single and Double FISH Protocols for Drosophila

   • Sarah C. Hughes, Henry M. Krause

   Pages 93-101

7. Confocal Microscopy of Plant Cells

   • Carol L. Wymer, Alison F. Beven, Kurt Boudonck, Clive W. Lloyd

   Pages 103-130

8. Preparation of Yeast Cells for Confocal Microscopy

   • Audrey L. Atkin

   Pages 131-139

9. Confocal Methods for Caenorhabditis elegans

   • Sarah L. Crittenden, Judith Kimble

   Pages 141-151

10. Imaging Sea Urchin Fertilization

    • Jon M. Holy

    Pages 153-166

11. Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy

    • James A. Langeland

    Pages 167-172

12. Confocal Microscopy on Xenopus laevis Oocytes and Embryos

    • Denise L. Robb, Chris Wylie

    Pages 173-183

13. Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos

    • Mark S. Cooper, Leonard A. D’Amico, Clarissa A. Henry

    Pages 185-204

14. Confocal Imaging of Living Cells in Intact Embryos

    • Paul M. Kulesa, Scott E. Fraser

    Pages 205-222

15. Live Confocal Analysis with Fluorescently Labeled Proteins

    • Helen Francis-Lang, Jonathan Minden, William Sullivan, Karen Oegema

    Pages 223-239

16. Live Imaging with Green Fluorescent Protein

    • Jim Haseloff, Emma-Louise Dormand, Andrea H. Brand

    Pages 241-259

17. Fluorescent Calcium Indicators:Subcellular Behavior and Use in Confocal Imaging

    • Donald M. O’Malley, Barry J. Burbach, Paul R. Adams

    Pages 261-303

18. Intracellular pH and pCa Measurement

    • Michal Opas, Ewa Dziak

    Pages 305-313

19. Measuring Dynamic Cell Volume In Situ by Confocal Microscopy

    • Rachel J. Errington, Nick S. White

    Pages 315-340

20. Imaging Thick Tissues with Confocal Microscopy

    • Delphine Imbert, Janet Hoogstraate, Emmeline Marttin, Christopher Cullander

    Pages 341-355

In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish.

Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.

"This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology

"...almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely...The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International

" This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes...there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today

"...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations..."-Ultramicroscopy

"...excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology

• Department of Molecular Biology, University of Wisconsin, Madison

  Stephen W. Paddock

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