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Bioluminescence Methods and Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Robert A. LaRossa

    1. DuPont Co., Wilmington

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Part of the book series: Methods in Molecular Biology (MIMB, volume 102)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-x
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  2. The Basics

    1. Front Matter

      Pages 1-1
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    2. Improvements in the Application of Firefly Luciferase Assays

      • Sharon R. Ford, Franklin R. Leach
      Pages 3-20

    3. Visualization of Bioluminescence

      • Amy Cheng Vollmer
      Pages 21-31

    4. Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone

      • Steven R. Kain, Guohong Zhang, Vanessa Gurtu, Paul A. Kitts
      Pages 33-42

  3. Analytical Biochemistry

    1. Front Matter

      Pages 43-43
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    2. Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue

      • Eberhard Jüngling, Helmut Kammermeier, Yvan Fischer
      Pages 45-53

    3. Bioluminescent Assay of the Guanylates

      • Sharon R. Ford, Franklin R. Leach
      Pages 55-68

    4. Bioluminescent Assay of the Adenylate Energy Charge

      • Sharon R. Ford, Franklin R. Leach
      Pages 69-81

  4. Molecular Biology Tools

    1. Front Matter

      Pages 83-83
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    2. Photorhabdus luminescens luxCDABE Promoter Probe Vectors

      • Tina K. Van Dyk, Reinhardt A. Rosson
      Pages 85-95

    3. Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome

      • David A. Elsemore
      Pages 97-104

    4. Probing for Promoters with Luciferase-Transposons

      • David C. Alexander, Michael S. DuBow
      Pages 105-122

    5. Cryopreservation and Reawakening

      • L. Winona Wagner, Tina K. Van Dyk
      Pages 123-127

    6. Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists

      • Vanitha Thulasiraman, Robert L. Matts
      Pages 129-141

  5. Cell-Based Assays

    1. Front Matter

      Pages 143-143
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    2. Genotoxic Sensors

      • Amy Cheng Vollmer
      Pages 145-151

    3. Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response

      • Tina K. Van Dyk
      Pages 153-160

    4. Real-Time Reporter of Protein Synthesis Inhibition

      • Matti Korpela, Marko Virta, Matti Karp
      Pages 161-168

    5. Luminescence-Based Cell Viability Testing

      • Ian A. Cree
      Pages 169-177
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About this book

The biological emission of light has fascinated humankind for mill- nia; who is not enchanted by the flashing of fireflies during summer's e- nings? Their collection by children in the area surrounding Baltimore, Maryland in the middle of this century for the purpose of biochemical isolation and ch- acterization of light producing proteins and cofactors may constitute the i- tiation of serious, quantitative studies of this phenomenon. The biochemistry of light production is certainly well-understood today. In bacterial systems it is coupled to the reducing and energy states of the cell, whereas in eukaryotic systems it is dependent upon the availability of ATP. These differences are reflected in the unrelated substrate requirements and structures of eukaryotic and prokaryotic luciferases. These biochemical studies have been aided by the cloning of luciferase-encoding structural genes, the structural genes for cofactor generation, and genes encoding proteins that interact with these lig- producing enzymes. These genes also provide starting materials for a wide range of applications that are the subject of this volume. Many of these advances have earlier been catalogued in two volumes of the Methods in Enzymology series. It is thus necessary to provide a rationale for the publication of Bioluminescence Methods and Protocols. The format of the Methods in Molecular Biology series differs significantly from that of the above-mentioned, classic compilation.

Reviews

". . . this book is a good review of the use of recombinant DNA technology associated with bioluminescence methods."-Analyst

Editors and Affiliations

  • DuPont Co., Wilmington

    Robert A. LaRossa

Bibliographic Information

  • Book Title: Bioluminescence Methods and Protocols

  • Editors: Robert A. LaRossa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896035204

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-577-8Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 310

  • Number of Illustrations: 33 b/w illustrations

  • Topics: Biochemistry, general

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