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  • © 1998

Cytochrome P450 Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 107)

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Table of contents (44 protocols)

  1. Front Matter

    Pages i-xiv
  2. Cytochrome P450 Nomenclature

    • David R. Nelson
    Pages 15-24
  3. Spectral Analyses of Cytochromes P450

    • John B. Schenkman, Ingela Jansson
    Pages 25-34
  4. Purification of Cytochromes P450

    • F. Peter Guengerich, Martha V. Martin
    Pages 35-54
  5. Purification of Extrahepatic Cytochromes P450

    • Margaret Warner, Jan-Ã…ke Gustafsson
    Pages 69-76
  6. Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems

    • F. Peter Guengerich, Natalie A. Hosea, Martha V. Martin
    Pages 77-84
  7. Cytochrome P450 Reconstitution Systems

    • Tsutomu Shimada, Hiroshi Yamazaki
    Pages 85-94
  8. Catalytic Assays for Human Cytochrome P450

    • Thomas K. H. Chang, David J. Waxman
    Pages 95-102
  9. Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation

    • David J. Waxman, Thomas K. H. Chang
    Pages 111-116
  10. High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6α-Hydroxylation

    • Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 123-128
  11. Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation

    • Thomas K. H. Chang, Charles L. Crespi, David J. Waxman
    Pages 147-152

About this book

In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques.

Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.



Editors and Affiliations

  • Department of Biochemistry, Queen Mary and Westfie1d College, University of London, UK

    Ian R. Phillips

  • Department of Biochemistry and Molecular Biology, University College, London, UK

    Elizabeth A. Shephard

Bibliographic Information

  • Book Title: Cytochrome P450 Protocols

  • Editors: Ian R. Phillips, Elizabeth A. Shephard

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896035190

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-580-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 482

  • Number of Illustrations: 49 b/w illustrations

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access