Gene Therapy Protocols

1. Front Matter

   Pages i-xiv

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2. Methods for Gene Transfer Using DNA-Adenovirus Conjugates

   • David T. Curiel

   Pages 1-23

3. Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors

   • Jeffrey S. Bartlett, Richard J. Samulski

   Pages 25-40

4. Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection

   • Warren S. Pear, Martin L. Scott, Garry P. Nolan

   Pages 41-57

5. Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers

   • Isabelle Rivière, Michel Sadelain

   Pages 59-78

6. Development of Replication-Defective Herpes Simplex Virus Vectors

   • David Krisky, Peggy Marconi, William F. Goins, Joseph C. Glorioso

   Pages 79-102

7. Methods for the Use of Poliovirus Vectors for Gene Delivery

   • Casey D. Morrow, David C. Ansardi, Donna C. Porter

   Pages 103-116

8. Methods for the Construction of Human Papillomavirus Vectors

   • Saleem A. Khan, Francis M. Sverdrup

   Pages 117-125

9. Methods for Liposome-Mediated Gene Transfer to the Arterial Wall

   • Elizabeth G. Nabel, Zhi-yong Yang, Hong San, Dianne P. Carr, Gary J. Nabel

   Pages 127-133

10. Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes

    • Mark A. Findeis, Catherine H. Wu, George Y. Wu

    Pages 135-152

11. Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium

    • John C. Olsen, Larry G. Johnson, James R. Yankaskas

    Pages 153-168

12. Methods for Adenovirus-Mediated Gene Transfer to Airway Epithelium

    • John F. Engelhardt

    Pages 169-184

13. Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung

    • Bruce R. Pitt, Margaret A. Schwarz, Richard D. Bland

    Pages 185-194

14. In Situ Retrovirus-Mediated Gene Transfer into the Liver

    • Nicolas Ferry, Sophie Branchereau, Jean-Michel Heard, Olivier Danos

    Pages 195-204

15. Methods for Delivery of Genes to Hepatocytes In Vivo Using Recombinant Adenovirus Vectors

    • Darlene Barr, Mark A. Kay

    Pages 205-212

16. Methods for Producing High Titer, Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cells

    • Jane C. Burns, Jiing-Kuan Yee, Alice L. Yu

    Pages 213-221

17. Methods for Efficient Retrovirus-Mediated Gene Transfer to Mouse Hematopoietic Stem Cells

    • John W. Belmont, Roland Jurecic

    Pages 223-240

18. Methods for Retrovirus-Mediated Gene Transfer into Primary T-Lymphocytes

    • Michel Sadelain

    Pages 241-248

19. Methods for Retrovirus-Mediated Gene Transfer to CD34+ Enriched Cells

    • Alfred B. Bahnson, Maya Nimgaonkar, Edward D. Ball, John A. Barranger

    Pages 249-263

20. Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy

    • Sabine A. Eming, Jeffrey R. Morgan

    Pages 265-279

In the last few years, significant advances have been made in the area of gene therapy for both genetic and acquired diseases. Improvement in gene transfer methods has allowed for development of gene therapy protocols for the treatment of diverse types of diseases, including metabolic, cardiovas- lar, and autoimmune diseases, as well as cancer. For example, clinical trials for gene therapy of cancer, cystic fibrosis, ADA deficiency, and arthritis, among others, have been initiated in recent years. It is likely that, in the near future, gene therapy will become a common form of treatment for many different types of diseases. Gene therapy takes advantage of recent advances in many areas of molecular and cell biology, including the identification of new the- peutic genes, improvement in both viral and nonviral gene delivery systems, better understanding of gene regulation, and improvement in cell isolation and transplantation. Because of the different and complex techniques involved in achieving successful gene-mediated therapies, it is difficult for scientists to perform all methods required for gene delivery and subsequent in vivo gene expression. This Gene Therapy Protocols volume in the Methods in Molecular Me- cine series provides scientists with detailed methods for working with gene transfer systems, and methods for gene transfer to specific tissue types either in vivo or ex vivo.

Such a compilation is of great interest fro researchers who get interested in the protocols utilized in gene transfer and in thier applications. Furthermore, for researchers already involved in gene therapy, this book is a very good reference with a "note section" containing many details and comments, useful to improve current protocols.-ACTA Neurologica Belgica

• University of Pittsburgh, USA

  Paul D. Robbins

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