Diagnostic Virology Protocols

1. Front Matter

   Pages i-xiii

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2. Rapid Detection of Adenovirus from Fecal Specimens

   • Tanvir Tabish, Alan Warnes, Stuart Clark

   Pages 1-6

3. Alphaviruses

   • John T. Roehrig, Teresa M. Brown, Alison J. Johnson, Nick Karabatsos, Denise A. Martin, Carl J. Mitchell et al.

   Pages 7-18

4. Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR

   • Xi Jiang, David O. Matson

   Pages 19-27

5. Enteroviruses and Rhinoviruses

   • Peter Muir

   Pages 29-50

6. Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction

   • Dennis W. Trent, Gwong-Jen Chang, A. Vance Vorndam, Robert S. Lanciotti

   Pages 51-61

7. Hepatitis Viruses

   • Girish J. Kotwal

   Pages 63-88

8. Herpesviruses

   • Mark J. Espy, P. Shawn Mitchell, David H. Persing, Thomas F. Smith

   Pages 89-101

9. Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper

   • Sharon Cassol, Stanley Read, Bruce G. Weniger, Richard Pilon, Barbara Leung, Theresa Mo

   Pages 103-117

10. PCR for the Detection of Influenza Viruses in Clinical Material

    • Joanna S. Ellis, David W. G. Brown

    Pages 119-127

11. Lyssaviruses

    • Hervé Bourhy

    Pages 129-142

12. Methods for Detecting Antimeasles, Mumps, and Rubella Virus Antibodies

    • Ilkka Julkunen, Irja Davidkin, Christian Oker-Blom

    Pages 143-157

13. Papillomaviruses

    • Sandra H. Kirk, David T. Y. Liu

    Pages 159-171

14. Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization

    • Karen E. Hicks, Stuart Beard, Bernard J. Cohen, Jonathan P. Clewley

    Pages 173-188

15. Polioviruses

    • David J. Wood

    Pages 189-197

16. Poxviruses

    • Hermann Meyer, Susan L. Ropp, Joseph J. Esposito

    Pages 199-211

17. Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections

    • G. L.

    Pages 213-222

18. Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis

    • Lennart Svensson

    Pages 223-229

19. Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNA by Calorimetric Enzymatic Immunoassay

    • M. Sofi Ibrahim, Connie S. Schmaljohn

    Pages 231-243

20. Time-Resolved Fluorescence

    • Pekka Halonen, Timo Lövgren

    Pages 245-255

The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

". . .an invaluable source of information for clinical and non-clinical virologists and microbiologists. . .would be a good asset to most research laboratories. . .For those that do perform these assays, it is invaluable."-Doody's Health Sciences Book Review Journal

• London School of Hygiene and Tropical Medicine, University of London, UK

  John R. Stephenson

• Micron Bioproducts, Camberley, UK

  Alan Warnes

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