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Table of contents (20 chapters)
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Front Matter
Keywords
About this book
This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.
Reviews
The authors...lay strong laboratory background for a wide variety of studies. This approach makes the book a pleasure to read, besides its immediate value as a reference source for routine work. The book is indispensable for every project in neuroscience. Four Stars! -Doody's Health Sciences Book Review Journal
Editors and Affiliations
Bibliographic Information
Book Title: Protocols for Neural Cell Culture
Editors: Sergey Fedoroff, Arleen Richardson
DOI: https://doi.org/10.1007/978-1-4757-2586-5
Publisher: Humana Totowa, NJ
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eBook Packages: Springer Book Archive
Copyright Information: Springer Science+Business Media New York 1997
eBook ISBN: 978-1-4757-2586-5Published: 17 April 2013
Edition Number: 2
Number of Pages: XX, 278
Number of Illustrations: 27 b/w illustrations
Topics: Neurosciences