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Apoptosis Techniques and Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Judes Poirier

    1. Douglas Hospital Research Centre, Verdun, Canada

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Part of the book series: Neuromethods (NM, volume 29)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Gene Tools to Study Neuronal Apoptosis

    • Isabelle Martinou, Harald Frankowski, Marc Missotten, Remy Sadoul, Jean-Claude Martinou
    Pages 1-11

  3. Detection of Apoptotic or Necrotic Death in Neuronal Cells by Morphological, Biochemical, and Molecular Analysis

    • Martin Gschwind, Gerda Huber
    Pages 13-31

  4. Detection of Cell Death in Neural Tissue and Cell Culture

    • Thomas Mahalik, Malcolm Wood, Gregory Owens
    Pages 33-46

  5. Cultured Cerebellar Granule Neurons as a Model of Neuronal Apoptosis

    • Guang-Mei Yan, Steven M. Paul
    Pages 47-66

  6. Optimization and Validation of RT-PCR as a Tool to Analyze Gene Expression During Apoptosis

    • Steven Estus
    Pages 67-84

  7. p53 Induction Is a Marker of Neuronal Apoptosis in the Central Nervous System

    • Shahin Sakhi, Steven S. Schreiber
    Pages 85-98

  8. Apoptosis and Ischemia in the Central Nervous System

    • Matthew D. Linnik
    Pages 99-132

  9. Analyses of Apoptosis- Associated DNA Fragmentation In Vivo During Neurodegeneration of the Peripheral Olfactory System in Adult Mammals

    • Emmanuel Moyse, Denis Michel
    Pages 133-159

  10. Both Irreversible Neuronal Death and Reversible Neuronal Stress Are Associated with Increased Levels of Statin, a Marker of Cell Cycle Arrest

    • Patricia Boksa, Uwe Beffert, Doris Dea, Richard Alonso, Dayan O’Donnell, Judes Poirier
    Pages 161-182

  11. β-Amyloid-Induced Neuronal Cell Death in Transgenic Mice and Alzheimer’s Disease

    • Frank M. LaFerla, Gilbert Jay
    Pages 183-195

  12. Use of TUNEL to Measure Retinal Ganglion Cell Apoptosis

    • Lisa Ayn Kerrigan, Donald J. Zack
    Pages 197-215

  13. Methods for Detection of Apoptosis in the CNS

    • Kathrin D. Geiger, Floyd E. Bloom, Nora E. Sarvetnick
    Pages 217-235

  14. Techniques for Distinguishing Apoptosis from Necrosis in Cerebrocortical and Cerebellar Neurons

    • Emanuela Bonfoco, Maria Ankarcrona, Dimitri Krainc, Pierluigi Nicotera, Stuart A. Lipton
    Pages 237-253

  15. The Nematode Caenorhabditis elegans as a Model System to Study Neuronal Cell Death

    • Paul Desjardins, Stephane Ledoux
    Pages 255-277

  16. Back Matter

    Pages 279-286
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About this book

After many years of neglect, the study of normal cell death and the so-called programmed cell death has finally gained some needed momentum. Evidence obtained over the last five years indicates that programmed cell death (also referred to as apop tosis) occurs normally in most animal tissues at some we- defined stages of their development and/or maturation. The molecular mechanisms by which it is executed remains - known. However, key regulatory and metabolic steps have been identified in the apoptotic cascade and a central concept has progressively emerged: Apoptosis appears to occur by default in many different cell types unless suppressed by signals ori- nating from neighboring cells. As Martin Raff noted a few years ago, this molecular cascade probably imposes a form of social control on cell survival and cell death. Since our understanding of the apoptosis phenomena has grown significantly over the last few years, we have been - powered by recent technological advances to biochemically d- sect the key metabolic components of apoptotic and necrotic cascades in normal and injured brains. Moreover, the devel- ment of improved histochemical tools has allowed us to better define the fundamental criteria of cell death, and transgenic te- nology has allowed scientists to test working hypotheses p- taining to the mechanism of action of the so-called “cell death gene.

Reviews

The book is useful for an actively working neuroscientist who is going to study apotosis and has enough experience to choose the most suitable models and protocols among all the diverse offerings of the book. Three Stars. -Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Douglas Hospital Research Centre, Verdun, Canada

    Judes Poirier

Bibliographic Information

  • Book Title: Apoptosis Techniques and Protocols

  • Editors: Judes Poirier

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1385/0896034518

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1997

  • eBook ISBN: 978-1-59259-634-8Published: 06 February 2008

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XI, 286

  • Topics: Neurosciences

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