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Drug'DNA Interaction Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Keith R. Fox

    1. University of Southampton, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 90)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. DNase I Footprinting

    • Keith R. Fox
    Pages 1-22

  3. Quantitative DNA Footprinting

    • James C. Dabrowiak, Jerry Goodisman, Brian Ward
    Pages 23-42

  4. Uranyl Photoprobing of DNA Structures and Drug–DNA Complexes

    • Niels Erik Møllegaard, Peter E. Nielsen
    Pages 43-49

  5. Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro

    • Christian Bailly, Michael J. Waring
    Pages 51-79

  6. Footprinting Studies with Nucleosome-Bound DNA

    • Philip M. Brown, Keith R. Fox
    Pages 81-93

  7. A Gel Mobility Shift Assay for Probing the Effect of Drug–DNA Adducts on DNA-Binding Proteins

    • Suzanne M. Cutts, Andrew Masta, Con Panousis, Peter G. Parsons, Richard A. Sturm, Don R. Phillips
    Pages 95-106

  8. An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug–Binding Sites

    • Suzanne M. Cutts, Con Panousis, Andrew Masta, Don R. Phillips
    Pages 107-115

  9. DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug–DNA Interactions

    • Thomas P. Dooley, Katherine L. Weiland
    Pages 117-125

  10. Transcriptional Footprinting of Drug–DNA Interactions

    • Don R. Phillips, Carleen Cullinane
    Pages 127-145

  11. Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays

    • John A. Hartley, Michael D. Wyatt
    Pages 147-156

  12. PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells

    • Keith A. Grimaldi, John A. Hartley
    Pages 157-180

  13. Analysis of DNA-Binding Antibodies

    • Jeremy S. Lee, Laura J. P. Latimer, Jamshid Tanha
    Pages 181-188

  14. Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies

    • Jeremy S. Lee, Laura J. P. Latimer, Gary D. Burkholder
    Pages 189-193

  15. Optical Absorbance and Fluorescence Techniques for Measuring DNA–Drug Interactions

    • Terence C. Jenkins
    Pages 195-218

  16. Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves

    • W. David Wilson, Farial A. Tanious, Maria Fernandez-Saiz, C. Ted Rigl
    Pages 219-240

  17. Electric Dichroism

    • Dietmar Porschke
    Pages 241-258

  18. Calorimetric Techniques for Studying Drug–DNA Interactions

    • Harry P. Hopkins Jr.
    Pages 259-268

  19. Methods for the Studies of Drug Dissociation from DNA

    • Fu-Ming Chen
    Pages 269-274

  20. Back Matter

    Pages 275-278
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About this book

In Drug-DNA Interactions, expert researchers describe powerful molecular techniques designed to illuminate and explore the interaction of drugs and ligands with DNA, often perfected in their own laboratories, . These easily reproducible methods involve sequence recognition properties, as well as physical approaches to measuring both the strength of interaction and the mode of drug binding to DNA. Drug interactions are also examined from a cellular perspective and for their usefulness in the development of new therapeutic agents.

Drug-DNA Interactions will be highly useful to all investigators needing the kind of detailed experimental and technical information often omitted from scientific papers. Those wishing to expand their repertoire of experimental techniques or to improve their current techniques will also find these powerful methods particularly helpful in elucidating the action of existing therapeutic agents, as well as in designing new anticancer drugs with improved action and reduced toxicity.






Reviews

"Methods in Molecular Biology is a long standing, respected series that provides protocols for the researcher. ..this particular book is a welcome addition for the researcher. It is recommended for all medical and science libraries."-Reviewer: H. Robert Malinowsky, E-STREAMS

Editors and Affiliations

  • University of Southampton, UK

    Keith R. Fox

Bibliographic Information

  • Book Title: Drug'DNA Interaction Protocols

  • Editors: Keith R. Fox

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/089603447X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1997

  • eBook ISBN: 978-1-59259-574-7Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Topics: Medicinal Chemistry

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