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  • © 1998

Enzyme and Microbial Biosensors

Techniques and Protocols

Part of the book series: Methods in Biotechnology (MIBT, volume 6)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
  2. Enzyme Biosensors

    1. Front Matter

      Pages 1-1
    2. Principles of Enzyme Biosensors

      • Ashok Mulchandani
      Pages 3-14
    3. Enzyme Biosensors Based on pH Electrode

      • Canh Tran-Minh
      Pages 15-22
    4. Enzyme Biosensors Based on Gas Electrodes

      • Marco Mascini, Gianna Marrazza
      Pages 23-34
    5. Enzyme Biosensors Based on ISFETs

      • Roland Ulber, Thomas Scheper
      Pages 35-50
    6. Enzyme Biosensors Based on Oxygen Detection

      • F. W. Scheller, D. Pfeiffer, F. Lisdat, C. Bauer, N. Gajovic
      Pages 51-65
    7. Enzyme Biosensors Based on Electron Transfer Between Electrode and Immobilized Peroxidases

      • Lo Gorton, Elisabeth Csöregi, Tautgirdas Ruzgas, Irina Gazaryan, György Marko-Varga
      Pages 93-120
    8. Enzyme Biosensors Based on Redox Polymers

      • Latha Shankar, Michael G. Garguilo, Adrian C. Michael
      Pages 121-132
    9. Enzyme Biosensors Based on Conducting Polymers

      • Wolfgang Schuhmann
      Pages 143-156
    10. Enzyme Sensors Based on Conductimetric Measurement

      • Norman F. Sheppard, Anthony Guiseppi-Elie
      Pages 157-173
    11. Enzyme Biosensors Based on Thermal Transducer/Thermistor

      • Kumaran Ramanathan, Masoud Khayyami, Bengt Danielsson
      Pages 175-186
  3. Microbial Biosensor

    1. Front Matter

      Pages 197-197
    2. Microbial biosensors based on respiratory inhibition

      • Yoshiko Arikawa, Kazunori Ikebukuro, Isao Karube
      Pages 225-235
    3. Microbial biosensors based on potentiometric detection

      • Aleksandr L. Simonian, Evgenia I. Rainina, James R. Wild
      Pages 237-248

About this book

In 1962 Clark and Lyons pioneered the concept of a biosensor. They p- posed immobilizing enzymes at electrochemical detectors to form “enzyme el- trodes” in order to expand the analyte range of ther base sensor. Smce then, the field of blosensors has greatly expanded. Some of the reasons for the expansion include both advances in signal transduction technologies and the incorporation of different biological sensing elements (Table 1). As a consequence, there are now a bewildering array of permutations of the biological sensing element and signal transducers that can be used to c- struct a biosensor. The purpose of the two volumes of Protocols and Te- niques in Biosensors is to provide a basic reference tool and starting point for use by graduate students, postdoctoral and senior researchers, and technicians m academics, industry, and government research establishments, to enable rapid entry into the field of biosensors. There are a variety of approaches that researchers employ to select a combination of bioaffinity elements and signal transducers. One commonly used approach is to identtfy the compound or compounds of interest; identify the biological molecule that yields an appropriate recognitionlselectivtty and dynamic concentration range for the assay; and choose an assay format and signal transduction technology that will meet the analytical requirements for the proposed application, This volume, Enzyme and Microbial Biosensors: Techniques and Protocols, describes a variety of transduction technologies that have been interfaced to enzymes and microorganisms.

Reviews

"Mulchandani and Rogers have assembled an international group of authors that presents a comprehensive panel of biosensors. . .the consistent format among chapters is useful and refreshing. . .chapters include concise descriptions of the fundamentals and developmental history of the highlighted transducer. . .every chapter includes tips for ensuring experimental success."-Molecular Biotechnology

Editors and Affiliations

  • University of California, Riverside

    Ashok Mulchandani

  • US-EPA, Las Vegas

    Kim R. Rogers

Bibliographic Information

  • Book Title: Enzyme and Microbial Biosensors

  • Book Subtitle: Techniques and Protocols

  • Editors: Ashok Mulchandani, Kim R. Rogers

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1385/0896034100

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-410-5Published: 17 April 1998

  • Softcover ISBN: 978-1-61737-028-1Published: 19 November 2010

  • eBook ISBN: 978-1-59259-484-9Published: 05 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XII, 264

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access