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Neurotransmitter Methods

  • Book
  • © 1997

Overview

Editors:
  1. Richard C. Rayne

    1. Birkbeck College, London, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 72)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Preparation of Brain Slices

    • Ting Wang, Ira S. Kass
    Pages 1-14

  3. Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method

    • Peter J. Bergold, Patrizia Casaccia-Bonnefil
    Pages 15-22

  4. The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotransmitter Action

    • Philip M. Larkman, Hrvoje Hećimović
    Pages 23-31

  5. Synaptosomes

    • Alexandra I. M. Breukel, Elly Besselsen, Wim E. J. M. Ghijsen
    Pages 33-47

  6. Synaptoneurosomes

    • Miriam N. G. Titulaer, Wim E. J. M. Ghijsen
    Pages 49-59

  7. Preparation of Synaptosomal Plasma Membranes by Subcellular Fractionation

    • Jacques J. H. Hens
    Pages 61-69

  8. Detection of Neuropeptides by Immunocytochemistry

    • Dick R. Nässel, Peter Ekström
    Pages 71-101

  9. Immunocytochemical Detection of Amino Acid Neurotransmitters in Paraformaldehyde-Fixed Tissues

    • David V. Pow
    Pages 103-123

  10. cGMP-Immunocytochemistry

    • Jan de Vente, Harry W. M. Steinbusch
    Pages 125-143

  11. Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue

    • Suzanna J. Newman, Stephen M. Gentleman
    Pages 145-152

  12. Localization of Nitric Oxide Synthase Using NADPH Diaphorase Histochemistry

    • Maurice R. Elphick
    Pages 153-158

  13. In Situ Hybridization to Determine the Expression of Peptide Neurotransmitters

    • Niovi Santama
    Pages 159-170

  14. Quantitative In Situ Hybridization Histochemistry

    • Andrew Levy
    Pages 171-183

  15. Microbore Liquid Chromatography Analysis of Monoamine Transmitters

    • Sophie Sarre, Katrien Thorré, Ilse Smolders, Yvette Michotte
    Pages 185-196

  16. Microbore Liquid Chromatography Analysis of Amino Acid Transmitters

    • Ilse Smolders, Sophie Sarre, Guy Ebinger, Yvette Michotte
    Pages 197-204

  17. HPLC Methods to Isolate Peptide Neurotransmitters

    • G. Mark Holman, Timothy K. Hayes
    Pages 205-218

  18. Neuropeptide Expression Patterns as Determined by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

    • Ka Wan Li, Wijnand P. M. Geraerts
    Pages 219-223

  19. GC/MS Determination of Biogenic Amines in insect Neurons

    • David G. Watson, Richard A. Baines, John M. Midgley, Jonathan P. Bacon
    Pages 225-237

  20. Monitoring Amino Acid Neurotransmitter Release in the Brain by In Vivo Microdialysis

    • Mark Zuiderwijk, Wim E. J. M. Ghijsen
    Pages 239-250
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About this book

Neurotransmitter Methods is intended as a bench-side companion for researchers who seek to identify, locaHze, or measure neurotransmitters and/or to identify sites of neurotransmitter action. Each method is detailed in a user-friendly "recipe" format and the protocols are accompanied by extensive notes to high­ light and explain crucial steps. Approaches utilizing an incredibly diverse array of modem techniques are presented: methods including HPLC, histochemistry, immunocytochemistry, in situ hybridization, mass spectrometry, microdialysis, and electrochemistry all make at least one appearance. In addition, protocols for associated methodologies, including the production of brain slices, dissociated neurons, synaptosomes/synaptoneurosomes, and neuronal plasma membranes are presented. Methods applicable to most of the recognized chemical types of neu­ rotransmitter are to be found and, although you may find absent any mention of your favorite neurotransmitter, many of the protocols are sufficiently general to be adapted to alternative uses. So, how does this book contribute usefiilly to the horde of methods volumes stampeding across our bookshelves? 1 hope that the strength oiNeurotransmitter Methods lies in the variety of its content. The book provides in a single volume an array of techniques that could take a researcher from selection and preparation of a tissue source through to identification and measurement of neurotransmitter content and even onto characterization of neurotransmitter sites of action. Furthermore, the diversity of approaches presented reflects the need for today's bench neuroscientist to be a bit of a "jack (or a "jill"!) of all trades.

Editors and Affiliations

  • Birkbeck College, London, UK

    Richard C. Rayne

Bibliographic Information

  • Book Title: Neurotransmitter Methods

  • Editors: Richard C. Rayne

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033945

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1997

  • Hardcover ISBN: 978-0-89603-394-8Published: 06 February 1997

  • Softcover ISBN: 978-1-61737-023-6Published: 10 November 2010

  • eBook ISBN: 978-1-59259-558-7Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 271

  • Topics: Neurosciences

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