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Epitope Mapping Protocols

  • Book
  • © 1996

Overview

Editors:
  1. Glenn E. Morris

    1. The North East Wales Institute, Wrexham, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 66)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Overview

    • Glenn E. Morris
    Pages 1-9

  3. Crystallographic Studies of Antigen-Antibody Interactions

    • Frederick A. Saul, Pedro M. Alzari
    Pages 11-23

  4. Epitope Mapping Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy

    • Irina Kustanovich, Anat Zvi
    Pages 25-37

  5. Mapping Epitopes on Antigens by Immunodiffusion in Gel

    • Giuseppe A. Molinaro, William C. Eby
    Pages 39-46

  6. A Simple Solid-Phase Competition Assay with Labeled Antigen

    • Masahide Kuroki
    Pages 47-53

  7. Epitope Mapping by Antibody Competition

    • Socrates J. Tzartos
    Pages 55-66

  8. Epitope Mapping by Surface Plasmon Resonance in the BIAcore

    • Berit Johne
    Pages 67-76

  9. Identifying Residues in Antigenic Determinants by Chemical Modification

    • N. Martin Young, Raymond P. Oomen
    Pages 77-83

  10. Epitope Mapping by Differential Chemical Modification of Antigens

    • Hans Rudolf Bosshard
    Pages 85-95

  11. Epitope Mapping by Proteolysis of Antigen-Antibody Complexes

    • Ronald Jemmerson
    Pages 97-108

  12. Proteolytic Fragmentation for Epitope Mapping

    • Maria R. Mazzoni, Nikolai O. Artemyev, Heidi E. Hamm
    Pages 109-120

  13. Epitope Mapping by Chemical Fragmentation

    • Glenn E. Morris
    Pages 121-127

  14. Probing Antibody-Antigen Interactions by Mass Spectrometry

    • Yingming Zhao, Brian T. Chait
    Pages 129-136

  15. Epitope Mapping Using Multipin Peptide Synthesis

    • Stuart J. Rodda, N. Joe Maeji, Gordon Tribbick
    Pages 137-147

  16. SPOT Synthesis

    • Ronald Frank, Heike Overwin
    Pages 149-169

  17. Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mapping Antigenic Determinants

    • Clemencia Pinilla, Jon R. Appel, Richard A. Houghten
    Pages 171-179

  18. Epitope Mapping Using Phage-Displayed Peptide Libraries

    • Diane Dottavio
    Pages 181-193

  19. Reiterative Screening of Phage-Display Peptide Libraries with Antibodies

    • Alexander Pereboev, Glenn E. Morris
    Pages 195-206

  20. Homolog Scanning

    • Lin-Fa Wang
    Pages 207-220
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About this book

Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo­ gists from a wide range of disciplines in which antibodies are used as molecu­ lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune diseases, or in defining protein topology in intact cells or organelles, to mention only a few of the possibilities. The aim of Epitope Mapping Protocols is to provide both a useful range of alternative practical methods for the experienced mapper and a fairly com­ prehensive introduction for someone embarking on antibody production and mapping for the first time. Contributors were encouraged to illustrate their protocols with results from their own research and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptide libraries; and the third group of eleven requires antigen expressed from recombinant DNA. Within this last group, methods more likely applicable to conformational epitopes are placed first.

Editors and Affiliations

  • The North East Wales Institute, Wrexham, UK

    Glenn E. Morris

Bibliographic Information

  • Book Title: Epitope Mapping Protocols

  • Editors: Glenn E. Morris

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033759

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1996

  • Softcover ISBN: 978-0-89603-375-7Published: 01 August 1996

  • eBook ISBN: 978-1-59259-552-5Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 416

  • Topics: Biochemistry, general

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