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Species Diagnostics Protocols

PCR and Other Nucleic Acid Methods

  • Book
  • © 1996

Overview

Editors:
  1. Justin P. Clapp

    1. Department of Biology, University of York, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 50)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation and Purification of Plant Nucleic Acids

    • Mien T. G. van de Ven, Patrick G. Lanham, Rex M. Brennan
    Pages 1-14

  3. Isolation and Purification of Insect DNA

    • Andrew F. Cockburn, Gary A. Fritz
    Pages 15-23

  4. Isolation and Purification of Vertebrate DNAs

    • David T. Bilton, Maarit Jaarola
    Pages 25-37

  5. Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies

    • William C. Black IV
    Pages 39-55

  6. Molecular Identification of Phytopathogenic Viruses

    • Donato Gallitelli, Pasquale Saldarelli
    Pages 57-79

  7. Improved PCR Methods for Identification of Phytopathogenic Viruses

    • Angelantonio Minafra, Donato Gallitelli
    Pages 81-91

  8. PCR Detection of HIV

    • Jürg Böni
    Pages 93-107

  9. The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV

    • Henk L. Smits
    Pages 109-126

  10. The Identification of Dengue Virus Using PCR

    • Kouichi Morita
    Pages 127-132

  11. The Design and Application of Ribosomal RNA-Targeted, Fluorescent Oligonucleotide Probes for the Identification of Endosymbionts in Protozoa

    • Nina Springer, Rudolf Amann, Wolfgang Ludwig
    Pages 133-144

  12. Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes

    • Anthony J. Bjourson, James E. Cooper
    Pages 145-154

  13. The Use of RAPD for Generating Specific DNA Probes for Microorganisms

    • Marco Bazzicalupo, Renato Fani
    Pages 155-175

  14. ITS-RFLP Matching for Identification of Fungi

    • Monique Gardes, Thomas D. Bruns
    Pages 177-186

  15. Specific PCR Primers for the Identification of Endomycorrhizal Fungi

    • Luc Simon
    Pages 187-192

  16. Single-Strand Conformational Polymorphism (SSCP) for the Identification of Endomycorrhizal Fungi

    • Luc Simon
    Pages 193-197

  17. The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi

    • Peter Wyss
    Pages 199-207

  18. Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe

    • Kesara Anamthawat-Jónsson, J. S. Pat Heslop-Harrison
    Pages 209-225

  19. Detection and Characterization of Leishmania Parasites by DNA-Based Methods

    • Guillaume J. J. M. Van Eys, Stefanie E. O. Meredith
    Pages 227-242

  20. The Molecular Identification of Trypanosomes

    • Geoff Hide
    Pages 243-262
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About this book

Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The majority of applications have been in such medically related fields as the diagnosis of disease or the identification of dis­ ease vectors, but this emphasis is changing as the potential and range of the techniques becomes more generally recognized. This has been especially true since the advent of polymerase chain reaction (PCR) technology. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification. It must be stressed, however, that conventional methods are still extremely valuable and will always remain at the forefront of many diagnostic procedures. The ability of the skilled taxonomist begins to fail when there is a requirement to identify rapidly organisms that are small, immature, or present in large numbers. In such cases the time scale necessary to obtain an identification often precludes practical usage. It is in this context that molecular methods come into their own. There are several advantages that DNA can offer diagnostics other than the obvious and important fact that its base sequence is the fundamental source of biological variation. It offers a great amount of information that is not accessible to other methods of identifica­ tion because the vast majority of DNA is not expressed.

Reviews

...a wonderful compilation of modern PCR-type species identification methods. Methods are clerly presented with illustrations as needed. This manual is a must for those using or considering the use of PCR as a mechanism of species identification.-SIM News

Editors and Affiliations

  • Department of Biology, University of York, UK

    Justin P. Clapp

Bibliographic Information

  • Book Title: Species Diagnostics Protocols

  • Book Subtitle: PCR and Other Nucleic Acid Methods

  • Editors: Justin P. Clapp

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033236

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1996

  • Softcover ISBN: 978-0-89603-323-8Published: 26 October 1995

  • eBook ISBN: 978-1-59259-537-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 416

  • Topics: Cell Biology

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