Skip to main content
SpringerLink
Log in
Book cover

Yeast Protocols

Methods in Cell and Molecular Biology

  • Book
  • © 1996

Overview

Editors:
  1. Ivor H. Evans

    1. School of Chemical and Life Sciences, University of Greenwich, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 53)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (40 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Isolation and Identification of Yeasts from Natural Habitats

    • John F. T. Spencer, Dorothy M. Spencer
    Pages 1-4

  3. Maintenance and Culture of Yeasts

    • John F. T. Spencer, Dorothy M. Spencer
    Pages 5-15

  4. Mutagenesis in Yeast

    • John F. T. Spencer, Dorothy M. Spencer
    Pages 17-38

  5. Rare-Mating and Cytoduction in Saccharomyces cerevisiae

    • John F. T. Spencer, Dorothy M. Spencer
    Pages 39-44

  6. Protoplast Fusion in Saccharomyces cerevisiae

    • Brendan P. G. Curran, Virginia C. Bugeja
    Pages 45-49

  7. Meiotic Analysis

    • John F. T. Spencer, Dorothy M. Spencer
    Pages 51-58

  8. Ascus Dissection

    • Carl Saunders-Singer
    Pages 59-67

  9. Karyotyping of Yeast Strains by Pulsed-Field Gel Electrophoresis

    • John R. Johnston
    Pages 69-78

  10. Chromosomal Localization of Genes Through Pulsed-Field Gel Electrophoresis Techniques

    • Graham R. Bignell, Ivor H. Evans
    Pages 79-88

  11. Isolation, Purification, and Analysis of Nuclear DNA in Yeast Taxonomy

    • Ann Vaughan-Martini, Alessandro Martini
    Pages 89-102

  12. Isolation of Yeast DNA

    • Peter Piper
    Pages 103-107

  13. Isolation of Mitochondrial DNA

    • Graham R. Bignell, Angela R. M. Miller, Ivor H. Evans
    Pages 109-116

  14. Isolation of Yeast Plasma Membranes

    • Barry Panaretou, Peter Piper
    Pages 117-121

  15. The Extraction and Analysis of Sterols from Yeast

    • Michael A. Quail, Steven L. Kelly
    Pages 123-131

  16. Peroxisome Isolation

    • Ben Distel, Inge van der Leij, Wilko Kos
    Pages 133-138

  17. Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers

    • Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield
    Pages 139-145

  18. Biolistic Transformation of Yeasts

    • Stephen A. Johnston, Michael J. DeVit
    Pages 147-153

  19. Genomic Yeast DNA Clone Banks

    • Graham R. Bignell, Ivor H. Evans
    Pages 155-187

  20. Yeast Colony Hybridization

    • Matthew John Kleinman
    Pages 189-192
Back to top

About this book

Yeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods—for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced—first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.

Editors and Affiliations

  • School of Chemical and Life Sciences, University of Greenwich, London, UK

    Ivor H. Evans

Bibliographic Information

  • Book Title: Yeast Protocols

  • Book Subtitle: Methods in Cell and Molecular Biology

  • Editors: Ivor H. Evans

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033198

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1996

  • eBook ISBN: 978-1-59259-540-2Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 433

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation