Yeast Protocols

1. Front Matter

   Pages i-xiii

   PDF

2. Isolation and Identification of Yeasts from Natural Habitats

   • John F. T. Spencer, Dorothy M. Spencer

   Pages 1-4

3. Maintenance and Culture of Yeasts

   • John F. T. Spencer, Dorothy M. Spencer

   Pages 5-15

4. Mutagenesis in Yeast

   • John F. T. Spencer, Dorothy M. Spencer

   Pages 17-38

5. Rare-Mating and Cytoduction in Saccharomyces cerevisiae

   • John F. T. Spencer, Dorothy M. Spencer

   Pages 39-44

6. Protoplast Fusion in Saccharomyces cerevisiae

   • Brendan P. G. Curran, Virginia C. Bugeja

   Pages 45-49

7. Meiotic Analysis

   • John F. T. Spencer, Dorothy M. Spencer

   Pages 51-58

8. Ascus Dissection

   • Carl Saunders-Singer

   Pages 59-67

9. Karyotyping of Yeast Strains by Pulsed-Field Gel Electrophoresis

   • John R. Johnston

   Pages 69-78

10. Chromosomal Localization of Genes Through Pulsed-Field Gel Electrophoresis Techniques

    • Graham R. Bignell, Ivor H. Evans

    Pages 79-88

11. Isolation, Purification, and Analysis of Nuclear DNA in Yeast Taxonomy

    • Ann Vaughan-Martini, Alessandro Martini

    Pages 89-102

12. Isolation of Yeast DNA

    • Peter Piper

    Pages 103-107

13. Isolation of Mitochondrial DNA

    • Graham R. Bignell, Angela R. M. Miller, Ivor H. Evans

    Pages 109-116

14. Isolation of Yeast Plasma Membranes

    • Barry Panaretou, Peter Piper

    Pages 117-121

15. The Extraction and Analysis of Sterols from Yeast

    • Michael A. Quail, Steven L. Kelly

    Pages 123-131

16. Peroxisome Isolation

    • Ben Distel, Inge van der Leij, Wilko Kos

    Pages 133-138

17. Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers

    • Robert C. Mount, Bernadette E. Jordan, Christopher Hadfield

    Pages 139-145

18. Biolistic Transformation of Yeasts

    • Stephen A. Johnston, Michael J. DeVit

    Pages 147-153

19. Genomic Yeast DNA Clone Banks

    • Graham R. Bignell, Ivor H. Evans

    Pages 155-187

20. Yeast Colony Hybridization

    • Matthew John Kleinman

    Pages 189-192

Yeast Protocols intends to offer a selection of well-proven proto­ cols in cell and molecular biology, applicable to yeasts including, but certainly not exclusively aimed at, Saccharomyces cerevisiae. Saccharomyces cerevisiae and its very distant cousin, Schizosacc- romyces pombe, are of course now foremost model eukaryotic organisms, and the focus of wide-ranging experimental studies, especially those using molecular genetic techniques. Many of the lat­ ter, such as DNA sequencing and in vitro mutagenesis, are general DNA techniques, well covered in other volumes of Humana's Meth­ ods in Molecular Biology series, and elsewhere. The inclusion of a number of non-DNA techniques in this book is meant to reflect the resurgent interest in yeast cell biology sparked by the development of gene manipulation methods—for example, cellular localization of cloned gene products using microscopical techniques. The presentation of protocols follows the successful Methods in Molecular Biology series format, with a clear sequence of steps and extensive troubleshooting notes. It is our hope that these protocols will be useful not only to established members of the full-time research community, but also to the less experienced—first degree level and masters students undertaking project work, as well as PhD students starting their experimental programs; I am well aware that these young apprentice scientists are not always able to receive the supervision time that they, and indeed their supervisors, would like.

• School of Chemical and Life Sciences, University of Greenwich, London, UK

  Ivor H. Evans

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