Capillary Electrophoresis Guidebook

1. Front Matter

   Pages i-ix

   PDF

2. General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation

   1. Fundamentals of Capillary Electrophoresis Theory

      • Kevin D. Altria

      Pages 3-13

   2. Standard Commercial Instrument Description

      • Kevin D. Altria

      Pages 15-19

   3. Typical Operating Procedures

      • Kevin D. Altria

      Pages 21-28

   4. Method Development/Optimization

      • Kevin D. Altria

      Pages 29-48

   5. Quantitation Procedures

      • Kevin D. Altria

      Pages 49-60

   6. Optimization of Precision in Quantitative Analysis

      • Kevin D. Altria

      Pages 61-70

   7. Optimization of Sensitivity

      • Kevin D. Altria

      Pages 71-81

   8. Method Validation

      • Kevin D. Altria

      Pages 83-97

   9. Fraction Collection

      • Kevin D. Altria

      Pages 99-103

   10. Troubleshooting

       • Kevin D. Altria

       Pages 105-118

   11. Quick Guide to Running a Successful Separation

       • Kevin D. Altria

       Pages 119-122

3. Applications of Capillary Electrophoresis and Specific Technologies

   1. Micellar Electrokinetic Chromatography

      • Koji Otsuka, Shigeru Terabe

      Pages 125-155

   2. Capillary Gel Electrophoresis

      • András Guttman

      Pages 157-169

   3. Chiral Separations by Capillary Electrophoresis

      • Manus M. Rogan, Kevin D. Altria

      Pages 171-196

   4. Capillary Electrochromatography

      • Iain H. Grant

      Pages 197-209

   5. Application and Limits of Sample Stacking in Capillary Electrophoresis

      • Dean S. Burgi, Ring-Ling Chien

      Pages 211-226

   6. Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis

      • Herbert E. Schwartz, Kathi J. Ulfelder

      Pages 227-264

   7. Application of Capillary Electrophoresis to Pharmaceutical Analysis

      • Kevin D. Altria

      Pages 265-284

   8. Separation of Peptides and Protein Digests by Capillary Electrophoresis

      • Marzell Herold, Gordon A. Ross, Rudolf Grimm, David N. Heiger

      Pages 285-308

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap­ idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro­ vided for individual analyses. The intention is to provide general guide­ lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth­ ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

The book is a very useful guidebook for CE practitioners as well as a reference book for researchers and graduate students. I highly recommend this book for libraries and individuals active in the field of CE.-FEBS Letters

• Glaxo Research and Development, Ware, Hertfordshire, UK

  Kevin D. Altria

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