Electroporation Protocols for Microorganisms

1. Front Matter

   Pages i-xix

   PDF

2. Electroporation Theory

   • James C. Weaver

   Pages 1-26

3. Instrumentation

   • Gunter A. Hofmann

   Pages 27-45

4. Direct Plasmid Transfer Between Bacterial Species and Electrocuring

   • Helen L. Withers

   Pages 47-54

5. Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation

   • Laura Gunn, Jennifer Whelden, Jac A. Nickoloff

   Pages 55-66

6. Production of cDNA Libraries by Electroporation

   • Christian E. Gruber

   Pages 67-79

7. Electroporation of RNA into Saccharomyces cerevisiae

   • Daniel R. Gallie

   Pages 81-91

8. Electrofusion of Yeast Protoplasts

   • Herbert Weber, Hermann Berg

   Pages 93-104

9. Escherichia coli Electrotransformation

   • Elizabeth M. Miller, Jac A. Nickoloff

   Pages 105-113

10. Electrotransformation in Salmonella

    • Kenneth E. Sanderson, P. Ronald MacLachlan, Andrew Hessel

    Pages 115-123

11. Electrotransformation of Pseudomonas

    • Jonathan J. Dennis, Pamela A. Sokol

    Pages 125-133

12. Electroporation of Xanthomonas

    • Teresa J. White, Carlos F. Gonzalez

    Pages 135-141

13. Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids

    • John R. McQuiston, Gerhardt G. Schurig, Nammalwar Sriranganathan, Stephen M. Boyle

    Pages 143-148

14. Electroporation of Francisella tularensis

    • Gerald S. Baron, Svetlana V. Myltseva, Francis E. Nano

    Pages 149-154

15. A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation

    • Hajime Hamashima, Makoto Iwasaki, Taketoshi Arai

    Pages 155-160

16. Genetic Transformation of Bacteroides spp. Using Electroporation

    • C. Jeffrey Smith

    Pages 161-169

17. Electrotransformation of Agrobacterium

    • Jhy-Jhu Lin

    Pages 171-178

18. Electroporation of Helicobacter pylori

    • Ellyn D. Segal

    Pages 179-184

19. Electrotransformation of Streptococci

    • Robert E. McLaughlin, Joseph J. Ferretti

    Pages 185-193

20. Transformation of Lactococcus by Electroporation

    • Helge Holo, Ingolf F. Nes

    Pages 195-199

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.

...the most comprehensive treatment available on the topic of electroporation of microorganisms...will be a useful resource for scientists working on diverse aspects of microbial genetics.-Four Stars, Doody's Health Sciences Book Review Journal

• Department of Cancer Biology, Harvard University School of Public Health, Boston

  Jac A. Nickoloff

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